Box-Behnken效应面法结合HPLC指纹图谱优选酒制太白山茱萸炮制工艺资料.docVIP

学院代号 10716 学 号 专业代码 1002 密 级 Shaanxi University of Chinese Medicine 学士学位论文 Box-Behnken效应面法结合HPLC指纹 图谱优选酒制太白山茱萸炮制工艺 学位申请人 专业名称 中药制药 申请学位类型 学士学位 指导教师姓名 论文提交日期 2008年7月 中文摘要 目的：研究太白山茱萸炮制工艺参数，设计响应面，并以马钱苷、莫诺干含量，结合指纹图谱优化其蒸制工艺条件。方法：采用高效液相色谱法，测定以山茱萸不同的蒸制工艺参数所得样品的图谱中马钱苷含量，莫诺苷含量结合指纹图谱为指标，通过3因素3水平的Box-Behnken响应面试验设计，考察加酒量、闷润时间、蒸制时间等因素对其炮制效果的影响；色谱柱 Diamonsil C18 （250 mm×4.6 mm，5 μm），柱温30 ℃；流动相 A：乙腈 B：0.1 %磷酸水溶液，梯度洗脱；流速为1.0 mL/min，Waters2996二极管阵列检测器，检测波长240 nm。结果：以全自动高效液相色谱法测定17批不同蒸制工艺的山茱萸药材谱图，经方法学考察和响应面设计，结果r=0.9998，莫诺苷在0.7141.666 mg/mL的范围内吸收度与浓度呈现良好的线性关系， r=0.9994马钱苷在0.06720.448 mg/mL的范围内吸收度与浓度呈现良好的线性关系，考察指标成分为马钱苷含量，莫诺苷含量，结合指纹图谱综合评定蒸制工艺。结论：本方法简洁、准确，易操作，酒蒸制山茱萸较清蒸工艺所得制品更优，且确定最佳加酒量为20 %，闷润时间为2 h，蒸制时间为6 h。 Box-Behnken效应面法。 Abstract Objective: To study the Taibai Mountain of cornel processing parameters, and loganin, Monod dry content, combined with the fingerprint of the steaming process condition optimization. Methods: by using high performance liquid chromatography determination of Cornus officinalis, with different steaming process parameters of sample from Atlas of loganin content, anthocyanin content with Monod fingerprint as index, by3factors and 3 levels Box-Behnken response surface experimental design, inspection, and a moistening time, steaming time and other factors on the processing effect; Diamonsil C18column (250 mm× 4.6 mm,5 μm ), the column temperature was 30 ℃; the mobile phase A: acetonitrile B:0.1 % water solution of phosphoric acid, gradient elution; the flow rate was 1.0mL/min, Waters2996 diode array detector, detection wavelength240nm. Results: a fully automated high-performance liquid chromatography method for the determination of the 20 batch of different steaming process of Cornus officinalis spectrum study, methodology, results r=0.9998, Monod was in the range of 0.714-1.666 mg/mL absorbance and concentration showed a good linear relationship, r=0.9994 loganin in the range of 0.0