Oglycosylation(o糖基化)讲述.pptxVIP

Introduction toGLYCOBIOLOGY;Chapter 5 O-linked Glycosylation;Mucin-type O-glycans (O-GalNAc) O-glycans in proteoglycan (O-Xyl, O-GalNAc) O-Fuc O-GlcNAc …… Play structural or signal roles;Linkage of Mucin-type O-Glycans to Proteins;5.1 Mucins are large, heavily O-glycosylated proteins holding water;Two portions: sugar and polypeptides;Mucin-type O-glycans with core 1 and core 2 structures;Mucin Polypeptides At least 19 human mucin genes have been distinguished by cDNA cloning. Mucin polypeptides: long, as many as 10,000 amino acids, polymorphic Tandem repeat (串联重复): 富含Ser和Thr, 序列具蛋白和种属特异性, 长10-80aa，在肽链分子中可多达百余拷贝 Unique sequences: specifying secretion, membrane insertion, oligomerization, and bacterial binding functions; further O- and N-glycosylated.;The amino- and carboxy-terminal regions are very lightly glycosylated, but rich in cysteines. The cysteine residues participate in establishing disulfide linkages within and among mucin monomers.;Schematic of Intestinal Secretory Mucin (MUC2);In addition to aiding in water retention, the mucins serve as lubrication and also help to protect from invasion by micro-organisms How to protect: Entrap potential pathogens; Some terminal domains have a specific affinity for bacterial cell surfaces, which can enhance entrapment and may also lead to immune responses against the bound organisms ;In addition to providing functions similar to those of the secreted mucins, the cell surface mucins can regulate adhesion between cells.;with mucin-like domain (tandem repeat);Decay accelerating factor (CD55,衰变加速因子);Cell surface mucins can mediate cell adhesion as a result of specific interactions of protein receptors with the O-linked sugar structures. Many such interactions result from unique terminal elaborations attached to the ends of polylactosamine chains on core 2 structures. Example: PSGL-1 (P-selectin glycoprotein ligand 1) The organization of PSGL-1 illustrates the dual role of the O-glycans in conferring physical p