2_genomics资料.pptVIP

/news_detail.php?news_id=1406 * /nature/journal/v443/n7109/full/443258a.html * www.liv.ac.uk/agf/454sequencing.html 454 Sequencing Facts ?How the 454 System works Attach DNA fragments to beadsThe system relies on fixing nebulized and adapter-ligated DNA fragments to small DNA-capture beads in a water-in-oil emulsion. Amplify the DNAThe DNA fixed to these beads is then amplified by PCR. Place beads in microscopic wellsEach DNA-bound bead is placed into a ~44 μm well on a PicoTiterPlate, which is a fiber optic chip. A mix of enzymes such as polymerase, ATP sulfurylase, and luciferase ar