Improvement of insect ecdysome on the hatching rate of .pptVIP

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2017-05-08 发布于北京
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Improvement of insect ecdysome on the hatching rate of .ppt

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Improvement of insect ecdysome on the hatching rate of .ppt

Improvement of insect ecdysome on the hatching rate of Artemia eggs Jia Qinxian1 Chen Lijing2 Zhen Mianping1 Content 1. Introduction 2.Treatment and Establish normal criterion method 3. Results 4. Conclusion 1.Introduction Concept： Diapause and Dormancy 1. Introduction:Understanding of Artemia eggs structure 1. Introduction:Understanding of Artemia eggs structure 1. Introduction:Study progress of Artemia eggs diapause (1)Structure 1.Introduction:Study progress of Artemia eggs diapause (2)Structure 1.Introduction:Study progress of Artemia eggs diapause (3)pHi 1. Introduction:Study progress of Artemia eggs diapause (4) Biotic active matter 1. Introduction:Study progress of Artemia eggs diapause (5)problem 1. Introduction:Some study progress of diapause in insects and other animal 2. Materials Artemia strains Chemical method: 3% H2O2 5min 10% NaClO 5min 5% NH4OH 5min 5% MgCl2 60min 5% CH3OH 30min 1% NaOH 5min 1M HCl 60min 5%CH2O 5min Physical method : -25℃freeze 4 to 20 weeks re-dehydrate 3 times Hormone: Insect ecdysome [2b, 3b, 14a, 22(R), 25- Pentahydroxy -7- cholesten-6-one] 2.Methods(1) Hormone treatment Concentration 0~90ppb，5~25ppm Dry Dipping Hormone Hatching eggs 180 min treatment 30~150 min time 2. Methods(2) Observation :dye cells/not dye cells by Gimmsa biotic pigment 3.Results -- (1) Establish normal criterion Comparison of dye/not dye cells of dry eggs* (1) Establish normal criterion Comparison of dye/not dye cells of wet eggs* (1) Establish normal criterion Hatching rate (%) and Dye cells/not Dye cells (1) Establish normal criterion Quality grading criterion 3. Results (2) Hormone treatment SHY strain (from Haiyang of Shangdong