FTI277对Nrf2和胃癌细胞增殖、凋亡的影响.pdf

摘要 摘 要 目的： 研究胃癌细胞中 FTI-277 对 Nrf-2 基因扩增及蛋白表达的影响及其对胃癌细 胞增殖、凋亡的作用。 方法： 采用 MTT 法观察当实验组中加入 FTI-277 后对胃癌细胞增殖活性的影响； 流式细胞仪观察 FTI-277 对胃癌细胞凋亡的影响。PCR、Western blot 法分别检 测实验组与对照组中 Nrf-2 基因扩增及蛋白的表达情况。 结果： FTI-277 对胃癌细胞增殖活性具有明显的抑制作用，且呈现出剂量依赖性。 FTI-277 具有诱导胃癌细胞凋亡的作用，荧光染色可见明显核碎裂、染色体聚集 等。加入 FTI-277 组 Nrf-2 的 mRNA 和蛋白的表达量均明显降低，与对照组相比 差异有统计学意义（p﹤0.01）。 结论： FTI-277 对胃癌细胞的增殖具有抑制作用、并且促进胃癌细胞自身凋亡。Ras 信号转导通路通过调节 Nrf-2 基因扩增及蛋白的表达。抑制胃癌细胞增殖，促进 胃癌细胞凋亡。 关键词：Nrf-2；胃癌；凋亡 II 万方数据 Abstract ABSTRACT Objective: We Researched the influence of FTI – 277 to the Nrf - 2 gene amplification and protein expression in the gastric cancer cells and its effect on gastric cancer cell proliferation and apoptosis. Methods: We used MTT method to observe the cells proliferation activity of gastric cancer cells when added FTI-277 in experimental group; Flow cytometry techniquewas used to observe the gastric cancer cell apoptosis when added FTI-277. PCR and Western blot method were used to test the Nrf -2 genes and protein expression in the experimental group and control group. Results: It was obvious that FTI-277 has an inhibitory effect on the proliferation activity of gastric cancer cells and presented a dose dependent. FTI-277 have the function of induced gastric cancer cell apoptosis, and there were obvious karyorrhexis, chromosome fluorescent dye aggregation, etc. the level of mRNA and expression of protein of Nrf-2 were significantly reduced when added FTI-277, which was statistically signif