05-第五讲_蛋白质相互作用.pptxVIP

第五讲 蛋白质相互作用;Protein-protein interactions determine the outcome of most cellular processes: DNA replication and transcription Protein synthesis and secretion Signal transduction Metabolism Cell cycle control ……;Qualitative methods in vivo: A) Yeast two-hybrid; B: Phage display; C) Co-IP Qualitative methods in vitro: A)GST pull-down; B) gel-filtration chromatography; C) Chemical cross-linking Quantitative methods in vitro: A) Fluorescence spectrometry; B) Surface Plasmon resonance (SPR);C) Isothermal Titration Calorimetry (ITC) Structural methods: A) NMR spectrometry ; B) X-ray crystallography