脂筏参与阿尔茨海默病的自噬溶体降解途径.doc

A non-invasive, rapid method to genotype late-onset Alzheimer’s disease-related apolipoprotein E gene polymorphisms Li Yi1, Ting Wu1, Wenyuan Luo1, Wen Zhou2,? Jun Wu1 1 Department of Neurology, Peking University Shenzhen Hospital, Shenzhen, Guangdong Province, China2 Department of Radiology, Peking University Shenzhen Hospital, Shenzhen, Guangdong Province, China 窗体顶端 Abstract: The apolipoprotein E gene ε4 allele is considered a negative factor for neural regeneration in late-onset Alzheimer’s disease cases. The aim of this study was to establish a non-invasive, rapid method to genotype apolipoprotein E gene polymorphisms. Genomic DNA from mouth swab specimens was extracted using magnetic nanoparticles, and genotyping was performed by real-time PCR using TaqMan-BHQ probes. Genotyping accuracy was validated by DNA sequencing. Our results demonstrate 100% correlation to DNA sequencing, indicating reliability of our protocol. Thus, the method we have developed for apolipoprotein E genotyping is accurate and reliable, and also suitable for genotyping large samples, which may help determine the role of the apolipoprotein E ε4 allele in neural regeneration in late-onset Alzheimer’s disease cases. Key words： nerve regeneration; neurodegeneration; late-onset Alzheimer’s disease; apolipoprotein E gene; real-time PCR; DNA sequencing; risk factor; allele; neural regeneration; 窗体底端 Acceptance date: 2013-12-14 Cite this article: Li Yi, Ting Wu, Wenyuan Luo, Wen Zhou, Jun Wu. A non-invasive, rapid method to genotype late-onset Alzheimer’s disease-related apolipoprotein E gene polymorphisms[J]. Neural Regeneration Research, 2014, 9 1 : 69-75.