NMNAT1对MES235帕金森病细胞模型CAT及TH蛋白表达影响.pdfVIP

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NMNAT1对MES235帕金森病细胞模型CAT及TH蛋白表达影响.pdf

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Experimental Study of Effect of Nicotinamide Mononucleotide Adenylyl Transferase 1 on Catalase and Tyrosine Hydroxylase in Cell Models of Parkinson’s Disease(PD)MES23．5 Cells Abstract Aim To investigate the effects of nicotinamide mononucleotide adenylyl transferase 1 (NMNAT 1)on the expression of catalase(CAT)and Tyrosine hydroxylase(TH)in cell Models of Parkinson’S disease fPDl MES23．5 cells．Methods Before Parkinsonian cell model establishment， synthetic NMNATl overexpression(NMNATl．0E1 vehicle plasmids，NMNATl overexpression plasmids．NMNATl siRNA vehicle plasmids and NMNATl siItNA(NMNATl IⅢAi)plasmids were transfected into MES23．5 cells using cell tranSfection techniques．Cells were divided into six groups：pEGFP group． pEGFP+MPP+ group， NMNATl-0E+MPP+ group，pMagic4．1，pMagic4．1+MPP+， NMNATl．RNAi+MPP+,The protein levels of Caspase 8 and TH in the six groups were measured by Western blots． Results Compared with DEGFP group，marked decreased in the protein 1evel ofCAT was observed in pEGFP+MPP+group(t=9．01．PO．001)．and TH expression decreased(t--0．32，p=0．0 1 2)．Compared with pEGFP+MPP+group．CAr expression increased(仁2．51．p-=0．033)and TH expression increased(t=0．45．p=0．002) in NMNATl-0E+MPP十group．Compared wi血pMagie4．1 control group．marked decrease in the protein level of CAT(t_2．47．p=0．036) and decrease in the protein level of TH(仁0．49。p=O．001) was observed in pMagie4．1+MPP+group．Compared埘th pMagic4．1+MPP+group，CAT expression decrease(t=3．53．p=0．006)and TH expression decreased(t--0．39,p=0．004)in NMNATl．I≈NAi+MPP+group．Conclusion Our results suggest that NMNAT 1 iS effective to up．regulate the expression of CAT which is one of antioxidant enzyme against oxidative injury and TH．Nmnatl may play an important role in protecting DA neurons through C蛆． Postgratude studente： Directed by Professor： De-Jie Du(Neuology) AU．MU Xie Keywords：NMNATl；Parkinson disease；apoptosis；Caspase8；TH 目录引言??????????．???????????．．1 第l章实验材料与方法????????????????．2 1实验材料????????????????????2 1．1 MES23．5细