检测大肠杆菌试纸条的制作要点.ppt

 Development of Colloidal Gold Test Strip for Detection of EHEC O157:H7 1、The cultivation of EHEC 0157: H7 2、McAb epitopes identification 3、Hybridoma recovery and McAb preparation 4、Preparation of Colloidal Gold Test Strip for Detection of EHEC O157:H7 5、Colloidal gold test strip performance evaluation 6、Market pork sample detection 1、The cultivation of EHEC 0157: H7 EHEC O157:H7 CT-SMAC 37℃ 18h mEC+n 37℃ 12h colony count Collect cells 100℃ water bath 30 min EHEC O157：H7 Antigen 1×109CFU/mL 4℃ 2、McAb epitopes identification 2.1 Determination of the optimal antigen coating concentrat