Science子刊揭示癌症、糖尿病新靶点研讨.docVIP

Name of journal: World Journal of Gastroenterology ESPS Manuscript NO: 11261 Columns: OBSERVATIONAL STUDY MicroRNA-103: A molecular link between insulin resistance and nonalcoholic fatty liver disease Xu Q et al. MicroRNA-103 links insulin resistance and NAFLD Qian Xu, Ying Li, Yong–Fang Shang, Hui-Ling Wang, Min-Xiu Yao Qian Xu, Ying Li, Yong–Fang Shang, Hui-Ling Wang, Min-Xiu Yao, Department of Endocrinology, the Second Affiliated Hospital of Medical College of Qingdao University, Qingdao 266042, Shandong Province, China Author contributions: Xu Q and Yao MX performed the majority of experiments, designed the study and wrote the manuscript; Shang YF, Li Y and Wang HL collected part the clinical materials. Correspondence to: Dr. Min-Xiu Yao, Department of Endocrinology, the Second Affiliated Hospital of Medical College of Qingdao University, 16 Jiangsu Road, Qingdao 266042, Shandong Province, China. yaominxiu@ Telephone: +86-532 Fax: +86-532 Received: May 12, 2014 Revised: June 20, 2014 Accepted: July 29, 2014 Published online: Abstract AIM: To investigate the associations between microRNA-103 (miR-103) and both insulin resistance and nonalcoholic fatty liver disease (NAFLD). METHODS: Serum samples were collected from 50 NAFLD patients who were overweight or obese (NAFLD group) and from 30 healthy subjects who served as controls (normal control group). Quantitative PCR was used to detect the expression of miR-103. Fasting plasma glucose, fasting insulin and triglyceride (TG) levels were measured. Homeostasis model assessment was used to evaluate basal insulin resistance (HOMA-IR). Patient height and weight were measured to calculate body mass index (BMI). RESULTS: Compared with the normal control group, higher levels of miR-103 in serum were expressed in the NAFLD group (8.18 ± 0.73 vs 4.22 ± 0.61, P = 0.000). When P = 0.01 (bilateral), miR-103 was positively correlated with HOMA-IR (r = 0.881), TG (r = 0.774) and BM