儿童期创伤对抑郁症患者糖皮质激素受体1F启动子区域CpG岛DNA甲基化状态影响.pdfVIP

Childhood trauma involving Glucocorticoid Receptor Gene Exon IF on CpG islands undergoing DNA methylation in Depression Abstract Background Maj or depression today，accounts as the leading cause of disability in the whole world affecting people of all ages．Many years of research on animal models and human models have documented the impact of early childhood experiences on the neurobiological mechanism affecting the psychopathology of late adult life．Children are dependent on the caregivers and parents for their basic needs such as physical，social and emotional needs．Eventually，these children undergo substantial developmental changes in their neural pathways that determine emotions and behaviour．The hypothalamus—pituitary—adrenal(HPA)axis plays a fflaj or role in the regulation of the stress responses of the human body． However changes occurring to the HPA axis may give rise to stress and risks for psychiatric disorders．Taking into consideration the multiple promoters of the GR，the promoter 1F in the CpG islands is more prone to methylation due to early life experiences．This study examines the status of DNA methylation occurring in the CpG islands of the GR promoter region 1 F in depressed patients with childhood trauma in China． Methods In order to avoid any bias and ensure the reliability and validity of this study，variables used for measuring childhood trauma and maj or depression were relatively adapted to the Chinese culture and the sampling design was also made to ensure a good representation of the Chinese population．60 subj ects(24 males and 3 6 females)were recruited II and divided into 3 groups of 20；namely healthy individuals as control， depressed patients with childhood trauma and depressed patients without childhood trauma．The depressed patients were given scores according to 5 different CTQ subscales namely emotional neglect and abuse，physical neglect and abuse，and sexual abuse．The blood samples obtained fiom a11 the subjects were used to examine the deg