Lipidomics.pptVIP

ESI Diluted sample go through the syringe pump through a hypodermic needle (5-20uL) A potential of 3-4kV is applied to the needle Heated nitrogen gas was used to help evaporating the solvent Small portion of the sprayed material enters a short capillary (0.2mm ID, 60mm length) that interfaces the atmospheric pressure spray to the first pumping stage. Most gaseous material is pumped off by a rotary vane pump as it exits the capillary. Some portion of the material enters the skimmer through the hole, and enters the high vacuum Mass Analyzer Types Triple Quad Q-Trap TOF (Time of flight) FT-