射频热凝对胶原酶化学溶解术影响体外实验研究.pdfVIP

摘要 ②射频热凝能够使椎问盘髓核变性，胶原纤维收缩。 ③射频热凝减少胶原酶对椎间盘髓核组织的溶解。 关键词：射频热凝；胶原酶；羟脯氨酸；糖胺多糖； 髓核；腰椎间盘 IIl Abs仃act ABSTRACT Ubjective： to study the effects of Radiofrequency thermocoagulation on Chemonucleolysis with collagenase by detection values of hydroxyproline and glycosaminoglycan absorbance and observed the changes of pathological structural for lumbar intervertebral disc nucleus pulposus Methods： Nucleus pulposus tissue(weighted for l g)voluntcrily donated by 24 patients with LDH and were collected with aseptic operation method，Then，111e specimen of nucleus pulposus were randomly assigned into 4 groups(n=6)：Radiofrequency group(group I)；collagenase group(group 10；radiofrequency collagenase group (group III)；the control group(group IV)．Standard radio frequency for Group I (temperature 90 o C，the time 120s X 2)，then Injection of saline 0．3 ml；injection of collagenase(50U，O．3m1)for group II；Given collagenase injection until the temperature drops to below 40。C after radiofrequency(RF parameters and dose of collagenase were unanimous、析m Group I and Group 111 for group III；blank control group(group IV)i画ection of saline 0．3 m1．All specimens in the sterile tube诵th saline 0．5ml(containing 0．01 mol／L CaCl2)，then Placed in a 37。C incubator after 24 hours．the specimen Was centrifuged，Measured by the spectrophotometer for value of hydroxyproline and proteoglycan in the supematant，and the precipitate after centrifugation for histological pathological examination． Results： ①The values of hydroxyproline absorbance for four groups were：0．096 4- O．021；0．549士O．021；0．314士0．112，0．087士0．040；the value ofgrouplI and grouplll were higher than group I and groupIV(P0．05)．the value of group II Was higher than grouplII(P0．05)，while no significant difference between group I and group IV(P= 0．86)． ②nle values ofhydroxyproline absorbance for four groups were：0．012-4-O．002； O．1 3 4-0．05 1；O．054 4-O．025；0．006士0．002．the value of grouplI and grouplll were Abstract higher than group I and grouplV(PO．05)．the value