高脂饮食对血管平滑肌细胞KATP通道功能的影响(精选).docVIP

高脂饮食对血管平滑肌细胞KATP通道功能的影响 　　作者：田红燕，范力宏，杨妙丽，胡 志，白晓君，马爱群，曹永孝 作者单位：西安交通大学医学院：1. 第一附属医院心内科，陕西西安 710061; 2. 第二附属医院耳鼻喉科，陕西西安 710004;3. 药理学系，陕西西安 710061 　　【摘要】 目的 观察高脂饮食对血管平滑肌的KATP通道功能的影响，探讨KATP通道在肥胖引起的可逆性高血压发病机制中的作用。方法 高脂饲料喂养大鼠，获得肥胖模型;20周后，其中10只转为普通饲料喂养，而另外10只继续喂养高脂饲料，实验继续10周。对照鼠为普通饲料喂养30周大鼠。30周时，处死大鼠，采用肌张力描记系统，膜片钳技术检测KATP通道的功能。结果 KATP通道电流及其介导的血管舒张反应在高脂鼠中明显降低，但转为普通饮食后，这些损伤被修复。结论 高脂饮食可引起大鼠血管平滑肌KATP通道功能的可逆性损伤，这可能是肥胖调节血压的一条潜在途径。 　　【关键词】 肥胖;KATP通道;肠系膜动脉;高血压;血管平滑肌细胞 　　BAI Xiaojun1, MA Aiqun1, CAO Yongxiao3 　　(1. Department of Cardiology, the First Affiliated Hospital, Medical School of 　　Xian Jiaotong University, Xian 710061; 2. ENT Department, the Second Affiliated Hospital, 　　Medical School of Xian Jiaotong University, Xian 710004; 3. Department of Pharmacology, 　　Medical School of Xian Jiaotong University, Xian 710061, China)ABSTRACT: Objective To investigate the effect of highfat dietinduced obesity on KATP channels in vascular smooth muscle cell (VSMC) and blood pressure. Methods Obesity was induced in rats by highfat diet for 20 weeks. Then these rats were divided into two groups. One group was fed with a highfat diet and the other group was fed with standard chow for 10 more weeks. Control rats were fed with standard laboratory rat chow. Function and expression of KATP channel in mesenteric arteries were characterized using myograghic system and patch clamp, respectively. Results The results indicated that highfat diet decreased function and expression of KATP channel in rat mesenteric arteries and increased blood pressure in rats. After conversion to a standard chow for 10 weeks, most of these effects were restored. Conclusion Highfat diet impaired KATP channel in VSMC, which may underscore reversible hypertension induced by highfat diet, at least in part. 　　KEY WORDS: obesity; KATP channel; mesenteric artery; hypertension; vascular smooth muscle cell 　　肥胖是高血压发生的一个重要危险因素，两者呈线性相关[1]。但是，肥胖导致高血压的具体机制还不清楚。血管平滑肌组织的KATP通道可