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HighefficiencytransformationoftallfescuewithAgrobacterium.ppt
High efficiency transformation of tall fescue withAgrobacterium tumefaciens Shujie Dong, Rongda Qu * Plant Science 168 (2005) 1453–1458 生科四甲 李廷原High efficiency transformation of tall fescue with Agrobacterium tumefaciens Plant Science 168 (2005) 1453–1458 Shujie Dong, Rongda Qu * Department of Crop Science, North Carolina State University, Raleigh, NC 27695-7620, USA Received 22 December 2004; accepted 14 January 2005 Available online 2 February 2005 Introduction Improvement on important agronomic traits, such as disease resistance and abiotic stress tolerance, would be very helpful for agronomic. Genetic engineering has opened new avenues to the modification,and provides us an alternative approach to meet specific breeding goals . Establishment of high frequency transformation system would greatly facilitate the efforts for grass improvement via genetic engineering. First attempts to introduce foreign DNA into tall fescue were through the protoplast and Agrobacterium tumefaciens-mediated transformation of tall fescue . Materials and methods Plant materials and tissue culture condition Agrobacterium strains and vectors Transformation procedure GUS histochemical assay and Southern analysis Inheritance study of the transgenic plant Materials and methods Plant materials and tissue culture condition Agrobacterium strains and vectors Transformation procedure GUS histochemical assay and Southern analysis Inheritance study of the transgenic plant Materials and methods Plant materials and tissue culture condition Agrobacterium strains and vectors Transformation procedure GUS histochemical assay and Southern analysis Inheritance study of the transgenic plant Materials and methods Plant materials and tissue culture condition Agrobacterium strains and vectors Transformation procedure GUS histochemical assay and Southern analysis Inheritance study of the transgenic plant Materials and methods Plant materials and tissue cul
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