甘草愈伤组织多合成调控.docVIP

甘草愈伤组织多糖合成调控 摘 要 ［目的］研究对胀果甘草愈伤组织中多糖类化合物的影响。［方法] MS培养基改变其蔗糖浓度，氮源类型比例和金属离子（Fe2+、Mn2+、 Zn2+）的浓度，在黑暗和光照下进行甘草愈伤组织培养，利用分光光度法检测甘草愈伤组织中多糖类化合物的含量。［结果］适合甘草愈伤组织生长的最佳条件是（24±1)oC光照培养，培养基为MS培养基基(附加1.0 mg/L 2，4-D和0.5 mg/L KT)，其中含有30 g/L 蔗糖、NH4+/NO3-为20/40、Fe2+0.05 mmol/L、Mn2+0.2 mmol/L、Zn2+0.3 mmol/L。适合甘草愈伤组织多糖积累的最佳条件是（24±1)oC光照培养，培养基为MS培养基基(附加1.0 mg/L 2，4-D和0.5 mg/L KT)，其中含有30 g/L蔗糖、NH4+/NO3-为20/40、Fe2+0 mmol/L、Mn2+mmol/L、Zn2+0.1 mmol/L。［结论］ Regulation of licorice polysaccharides in licorice callus ABSTRACT [Objective] To study the effects of the culture components and illumination on the accumulation of polysaccharide compounds . [Method] By adjusting the concentration of carbon, nitrogen and metalion（Fe2+、Mn2+、 Zn2+） in the MS culture solution, and kept the licorice callus under dark or illumination, then detected the content of polysaccharide compounds by spectrophotometry. [Result] The optimum condition of licorice callus growth was (24±1) oC in the light and the basic medium was Murashige and Skoog (MS) (plus 1.0 mg/L 2,4 - D and 0.5 mg/L KT) with 30 g/L sucrose, NH4+/NO3-为20/40、0.05 mmol/L Fe2+、0.2 mmolL Mn2+、 0.3 mmolL Zn2+.The optimum condition of licorice polysaccharides accumulation was (24±1) oC in the light and the basic medium was Murashige and Skoog (MS) (plus 1.0 mg/L 2,4 - D and 0.5 mg/L KT) with 30 g/L sucrose, NH4+/NO3- is 20/40、0 mmolL Fe2+、mmol/L Mn2+、0.1 mmolL Zn2+. [Conclusion] The optimal media of licorice callus growth and polysaccharides accumulation express well. Licorice as explant and with a view to an industrial production of polysaccharides by tissue and cell culture. Keywords: Licorice callus；Glycyrrhiza polysaccharide；Growth；polysaccharide 1 前言 1 1.1 甘草的简介 1 1.2 甘草多糖的简介 1 1.3 愈伤组织的培养 1 1.4 超声波法提取多糖 2 1.4.1 超声波提取技术 2 1.4.2 超声波萃取原理 2 1.4.3 超声波萃取的特点 3 1.5 苯酚-浓硫酸法测多糖含量 3 1.6 实验目的及意义 3 2 本论 5 2.1 材料与仪器 5 2.1.1 材料 5 2.1.2 仪器与试剂 5 2.2 实验方法 5 2.2.1 无菌材料的制备 5 2.2.2 愈伤组织诱导