肥胖性研究发表发表资料.ppt

Antioxidant and Neuronal Cell Protective Effects of Columbia Arabica Coffee with Different Roasting Conditions Functional Food Lab Guo Tianjiao Contents 1 Introduction 2 Materials and Methods 3 Results 4 Discussion Introduction Colombia arabica coffee (Coffea arabica) Coffee has been one of the most popular beverages all over the world. Coffee contains phenolic acids including caffeic acid, ferulic acid, and vanillic acid, and 5-O-caffeoylquinic acid. Coffee has beneficial effects on degenerative diseases including cancer , cardiovascular disorders. Alzheimer’s disease the hallmarks of Alzheimer’s disease neuro?brillar tangles bate-amyloid deposits Materials and Methods Sample preparation green beans medium (230°C, 10 min) → ,city (230°C, 12 min) ,french (230°C, 15 min) Roasted samples (2 g) were suspended and extracted with 100 mL of 70% ethanol at 70°C for 2 hr. Neuronal cell culture Neuronal PC12 cell (KCLB 21721, Korea Cell Line Bank, Seoul, Korea) ＋ cells respond reversibly to nerve growth factor (NGF) ↓ ABTS radical scavenging activity Ferric reducing antioxidant power (FRAP) Antioxidant 　　Fe3+-TPTZ —————— Fe2+-TPTZ (blue) (MDA) assay Determination of cell viability