E-ding-2010.pptVIP

Electron Microscopy of replicating DNA reveals replicating bubbles Priming is required to start DNA synthesis The cell cycle is controlled by many cyclin-Cdk complexes G1 S G2/M Cyclin-Cdk Cyclin-Cdk Cyclin-Cdk Budding Yeast CLN1,2,3-CDC28 CLB5,,(3,4)-CDC28 CLB1,2(3,4)-CDC28 Fission Yeast CIG1-CDC2 CIG2-CDC2 CIG13-CDC2 Higher Eukaryotes CyclinD1,2,3-CDK4/6 CyclinA-CDK2 CyclinB-CDC2 CyclinE1,2-CDK2 Inhibitory control of cell cycle PI染色检测细胞周期和细胞凋亡 Telomere replication Questions 1. Why replication is semi-conservative? What is the semi-conservative replication? Describe the structural and biological reasons for this replication mechanism? 2. What is semi-discontinuous replication？ Why DNA replication is semi-discontinuous? How the semi-discontinuous replication is accomplished in E. coli? 3. Compare the prokaryotic replication with eukaryotic replication. Questions 4. What is the end replication problem in eukaryotic cells? How is the end replication problem resolved 5. What are the mechanisms that keep the high accuracy of DNA replication 6. What is replicon? How many origins and termini shall a replicon contain? 7. Please describe the whole molecular process of bacterial DNA replication, including the featured steps and enzymes involved. Nuclear Matrix核基质 Scaffold(支架) of insoluble protein fibers which acts as an organizational framework for nuclear processing, including DNA replication, transcription.不溶性蛋白纤维组成的支架，作为核加工，包括DNA修复和转录的有组织的框架。 Replication factories 复制工厂（DNA and proteins) E4: Eukaryotic DNA replication Replication factories: all the replication enzymes, DNA associated with the replication forks in replication 复制工厂:所有的复制酶,与复制叉相关的DNA和蛋白质 BUdR labeled标记 Visualizing by immunof