益气化瘀法对大鼠肛周脓肿术后创面组织中VEGF表达的影响.docVIP

益气化瘀法对大鼠肛周脓肿术后创面组织中VEGF表达的影响 摘要：目的 观察益气化瘀法对大鼠肛周脓肿术后创面愈合过程中VEGF表达的影响，进一步探讨益气化瘀法促进创面愈合的作用机制。方法 将 30只SD大鼠完全随机分为模型组（10只）、对照组（10只）、治疗组（10）只，于术后第7 d，取创面全层组织，通过免疫组化检测创面组织中 VEGF含量表达水平和血管数，并观察创面愈合时间。结果 治疗组大鼠创面组织中 VEGF含量表达水平显著增加，与对照组、模型组比较，差异有统计学意义（P 0.001）。治疗组创面愈合时间明显优于模型组和对照组，差异有统计学意义（P 0.05）。结论 益气化瘀法明显促进实验大鼠肛周脓肿术后创面的愈合，通过上调VEGF表达，促进血管的新生，加速创面的愈合。 关键词：血管内皮生长因子；益气化瘀；创面修复；肛周脓肿 中图分类号：R285 文献标志码：A 文章编号：1007-2349（2016）06-0068-03 【Abstract】Objective： To observe the effect of Tonifying Qi to Dispering Blood Stasis Method on VEGF expression in postoperative wound tissue of rat perianal abscess and further explore its mechanism to promote wound healing. Methods： 30 SD rats were randomly divided into a model group（10）and a control group（10）and a treatment group（10）. In the seventh day after surgery，the wound full tissue of the rats was taken and VEGF content expression and number of blood vessels in the wound tissue were detected by immunohistochemistry and wound healing time was observed. Results： VEGF expression level in the wound tissue of the treatment group significantly increased. Compared with the control group and the model group，the difference was statistically significant （P 0.001）. The healing time of the treatment group was quicker than that of the model group and the control group，and the difference was statistically significant（P 0.05）. Conclusion： Tonifying Qi to Dispering Blood Stasis Method significantly promotes the postoperative wound healing of the rat perianal abscess and also promotes the rebirth of vascular and accelerates wound healing by upregulating the expression of VEGF. 【Key words】vascular endothelial growth factor，Tonifying Qi to Dispering Blood Stasis Method，wound healing，perianal abscess 肛周脓肿是肛肠科常见疾病，手术为主要治疗手段[1～2]，但术后由于特殊的解剖结构，加之粪便污染、炎症刺激等因素，创面的修复一直是研究的热点问题。中医益气化瘀法对慢性难愈性创面的修复有着较好的临床疗效。本研究旨在观察根据益气化瘀法自制生肌扶正油纱对实验大鼠肛周脓肿术后感染创面的促愈作用，并通过观察血管内皮生长因子（vascular factor，VEGF）在大鼠肛周脓肿术后创面愈合过程中的表达变化，进一步探讨与阐明益气化瘀法在创面愈合过程中的作用与机制。 1 材料与方法 1.1 实验材料 1.1.1 主要试剂 石蜡（上海国药集团座机电