20140526003氯化汞对鲤幼鱼鳃组织抗氧化系统和组织损伤1精读.docVIP

氯化汞对鲤幼鱼鳃组织抗氧化系统 和组织损伤研究 姜会民* 山东高校十二五规划重点实验室“动物生理与生化及应用”重点实验室，山东菏泽 274015 收稿日期：2014-05-26 录用日期：2014-07-09 摘要：在急性毒性试验的基础上，设定0.052 mg?、0.13 mg?、0.26 mg?三个汞离子染毒浓度和一个对照组研究无机汞污染对鲤鳃组织POD）活性和MDA含量以及鳃组织损伤的影响。实验结果显示：?L-1时POD活性显著的低于对照组（p 0.05）；实验组MDA含量均显著高于对照组（p 0.05）；实验组鳃组织可见鳃小片肿胀，基部增生，上皮细胞脱落，泌氯细胞增多，细胞核溶解及金属沉积。试验结果表明氯化汞对鲤鳃组织结构和抗氧化系统具有明显的损伤。 关键词：氯化汞；鳃；过氧化物酶；丙二醛；组织损伤 Effect of Mercury Chloride on Antioxidant System and Injury in Gill of Carps Jiang Huimin The Key Laboratory of Shandong University “the Application of Animal Physiology and Biochemistry ” Heze, Shandong Province 274015 China Received 26 May 2014 accepted 9 July 2014 Abstract: Based on the acute toxicity test,three mercury exposure concentration groups （0.052mg?L-1、0.13mg?L-1、0.26mg?L-1）and one control group were set to study the effect of mercury on activity of peroxidase(POD) and content of malondialdehyde (MDA) on gills as well as the injury of gill tissue. Results showed as follows: The activity of POD increased and then decreased with the concentration of mercury chloride increased,the activity of POD in SH group was significantly lower than that of the control group (p0.05);The content of MDA in experiment group was significantly higher than that of control group (p0.05 );The gill lamella of carps swelled, the base of gill lamella appeared hyperplasia,epithelial cells shed,the number of vacuolated cells increased, the cells of chloride increased, and the nucleus was dissolved,the deposition of mercury was visible. The results showed that mercury chloride has a significant effect on activities of antioxidant system and tissue injury in gill of carp. Key words: mercury chloride; gills; POD; MDA; injury 汞( ercury Hg)是一种广泛分布于环境中的剧毒金属 随着含汞农药在农业生产过程的使用，以及冶金、印染、木材防腐等含汞废水在工业生产中大量排放,造成了水环境中汞污染问题日益严峻[1]。特别是日本水俣病出现后，汞污染成为重金属污染物中的研究重点[]。已有的研究结果表明[3]水体中Hg2+的毒性及危害作用表现在水体中的Hg2+通过鱼类等水生生物富集体内积累，进而随食物链传递的生物放大作用，最后在人体内积累，对人体产生毒性作用。其毒性机理表现Hg2+与体内许多重要酶的活性中心巯基结合而抑制一系列含的酶的生理功能从而影响细胞的正常代谢功能[4]。Canli 研究发现[5]鳃是挪威