狗蔷薇类根体和类原球茎发生发育的组织学研究讲解.docVIP

园 艺 学 报 2014，41（6）：1167–1174 http: // www. ahs. ac. cn Acta Horticulturae Sinica E-mail: yuanyixuebao@126.com 收稿日期：2013–12–09；修回日期：2014–04–21 基金项目：国家自然科学基金项目（座机电话号码）；北京市教委2013年民办教育发展促进项目（民办892-34） * 通信作者 Author for correspondence（E-mail：zhaolj5073@） 狗蔷薇类根体和类原球茎发生发育的组织学研究 张建甫1,2，田传卫1，毕 玲1，赵梁军1,* （1中国农业大学观赏园艺与园林系，北京 100193；2北京城市学院城市建设学部，北京 100083） 摘 要：以狗蔷薇叶片为对象观察其类根体和类原球茎发生发育的过程。发现叶片外植体叶柄维管 束的原形成层细胞在2,4-D的诱导下分裂并扩大形成狭长的微管组织细胞，进一步衍生形成根源基细胞， 根源基细胞分裂形成根尖分生组织，并最终形成类根体。类根体具有根冠、生长点、伸长区和根毛区， 类根体只有初生结构，不具备次生结构。携带有愈伤组织的类根体在含TDZ的1/2MS培养基和光照协调 作用下，根尖分生组织消失并分化成成熟的薄壁细胞，中柱鞘上部的细胞形成胚性愈伤组织，其分化为 胚性细胞并形成新的分生中心，突破皮层和表皮，进而形成类原球茎。 关键词：狗蔷薇；叶片外植体；类根体；类原球茎；发生与发育；组织学研究；超微结构 中图分类号：S 685.12 文献标志码：A 文章编号：0513-353X（2014）06-1167-08 Studies on the Histology and Ultrastructure of the Initiation of Rhizoids and Protocorm-like Bodies（PLBs）Induced from the Leaf Explants of Rosa canina ZHANG Jian-fu1,2，TIAN Chuan-wei1，BI Ling1，and ZHAO Liang-jun1,* （1Department of Ornamental Horticulture and Landscape Architecture，China Agricultural University，Beijing 100193， China；2Department of Urban Construction，Beijing City University，Beijing 100083，China） Abstract：Leaf explants of Rosa canina were used to investigate the origins of the rhizoids and protocorm-like bodies formation. Histological studies revealed that remained procambium cells in the vascular bundles were induced by 2,4-D，then divided and enlarged to form long but narrow vascular tissue cells that functioned as pluripotent stem cells with a tendency to form root primordia which further developed to form root meristems. The rhizoids have the same anatomical structure with the roots growing in vitro，including root cap，root meristematic zone，elongation zone and maturation zone，and the rhizoids only has the primary structure not secondary structure. Incubating the rhizoids carrying calli under light/dark conditions on 1/2MS medium containing TDZ，the tips of rhizoids could develop into protocorm-like bodies（PLBs）. Histological