超声波法提取山楂叶中总黄酮条件研究论文解析.docVIP

本科生毕业设计（论文） 题 目：超声波法提取山楂叶中总黄酮的条件研究 2011级应用化学3班 指导教师： 　 2015年6月1日 摘 要山楂叶的化学成份复杂，从山楂叶中分离出的最多的功效组分是黄酮类物质利用法，以芦丁为参照品，硝酸铝作显色剂，在510nm波长测定总黄酮含量。结果测得工作曲线的回归方程为A=0.0091C+0.0023，r=0.9980，a=0.0023，b=0.0091。线性相关系数R=0.9980。不同提取、浓度、温度、时间、料液比总黄酮含量测定，乙醇70%，料液比1:20、温度50℃，时间60min137.555mg。通过四因素三水平的正交实验，得出影响总黄酮提取率的主要因素为乙醇浓度＞超声温度＞料液比＞超声时间关键词：山楂叶；黄酮；超声波法；分光光度法 ABSTRACT Refers to the total flavones, it is a kinds of natural products, widely exist in the plant kingdom, is many effective components of Chinese herbal medicine.Hawthorn complex chemical composition, isolated from hawthorn leaves most of the efficacy ingredient is flavonoids.This experiment using ultrasonic method，use Rutin as reference，Aluminum nitrate as chromogenic agent。The total content of flavonoids by spectrophotometry at the 510 wavelength.The results measured the regression equation of working curve for A=0.0091C+0.0023，r=0.9980，a=0.0023，b=0.0091。The linear correlation coefficient R=0.9980.By the method of single factor investigation, different extraction solvent, ethanol concentration, temperature, time, and solid-liquid ratio to determine the content of total flavonoids.Then get the better experimental conditions for:The volume fraction of ethanol 70%,?The material-water ratio1:20, Ultrasonic temperature 50℃,Ultrasonic time 60min, At this time of flavonoids content is 137.555mg.Through the four factors three levels orthogonal experiment get that the main factors influencing the flavonoids extraction rate is ethanol concentration ultrasonic temperature solid-liquid ratio ultrasonic time. Keywords：Hawthorn leaves; Flavonoids; Ultrasonic method; Spectrophotometry  第一章 绪论 1 1.1 研究背景 1 1.1.1 山楂叶的简介 1 1.1.2 山楂叶中黄酮类化合物 1 1.1.3 黄酮类化合物的研究现状 3 1.2 山楂叶中总黄酮的提取方法 4 1.2.1 水提法 4 1.2.2 浸渍法 4 1.2.3 微波提取法 5 1.2.4 酶解法 5 1.2.5 超临界流体萃取技术 5 1.2.6 超声波法 5 1.3 山楂叶中黄酮的测定方法 6 1.3.1 分光光度法 6 1.3.2 色谱法 6 1.3.3 纸色谱法 7 1.3.4 毛细管电泳法 7 1.4 总黄酮类化合物应用前景 7 1.4