FGFexpression.docVIP

Justin Gage Crump, et al 2004 . An essential role for Fgfs in endodermal pouch formation influences later craniofacial skeletal patterning. Development 131, 5703-5716 1,*, Lisa Maves1, Nathan D. Lawson2, Brant M. Weinstein3 and Charles B. Kimmel1 1Institute of Neuroscience, 1254 University of Oregon, Eugene, OR 97403-1254, USA 2Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA 3Laboratory of Molecular Genetics, NICHD/NIH, Bethesda, MD 20892, USA *Author for correspondence e-mail: gage@uoneuro.uoregon.edu Accepted 18 August 2004 用FGF受体抑制剂SU5402，显示FGF信号在体节早期阶段对第一个Pouch形成是需要的。在这个时期，FGF8在侧中胚层lateralmesoderm Reifers et al., 2000 表达，在中后脑交界midbrain-hindbrain boundary MHB 到R2和R4区域也有表达 Maves et al., 2002; Reifers et al., 1998 . 从4体节期（11.3hpf）开始，FGF3超过FGF8的表达 Maves et al., 2002; Walshe et al., 2002 , 中胚层和神经管FGF表达区域与发育中的咽中胚层相邻。在晚期，FGF3和FGF8（少量）在咽囊（pharyngeal pouches）中表达 David et al., 2002; Walshe and Mason, 2003a 。但我们用镶嵌分析显示，FGFs在恢复fgf8–; fgf3-MO斑马鱼咽弓结构，中胚层和神经管区域是必需，而咽中胚层不是。从而，我们发现了脑和头中胚层在控制咽中胚层发育成囊的过程中，FGF信号是必需的。 In addition to their requirement in pouch formation, we find that Fgf8 and Fgf3 have redundant, essential functions in pharyngeal cartilage development. In fgf8–; fgf3-MO animals, hyoid and branchial cartilages are largely absent and mandibular cartilages are reduced. By imaging pouch structure early and cartilage structure later in individual sides of animals in which Fgf signaling has been manipulated, we find that altered pouches correlate with later rearrangements of the cartilage pattern. This analysis suggests that pharyngeal pouch structure is a critical determinant of the pharyngeal cartilage pattern. We present a model in which an earlier function of Fgfs in pouch formation, in addition to their well-documented role as pouch-secreted survival factors later in development, contributes to the diversity of craniofacial phenotypes seen in fgf8 mutants. Phenotypic analysis细胞免疫化学，用了兔抗GFP Alcian Gree