NEB酶切buffer.docxVIP

HYPERLINK /nebecomm/default.asp? Home HYPERLINK /nebecomm/tech_reference/default.asp? Technical Reference HYPERLINK /nebecomm/tech_reference/restriction_enzymes/default.asp? Restriction Endonucleases NEBuffer Activity Chart NEBuffer Activity Chart for Restriction Enzymes For your convenience, New England Biolabs offers a simple 4 buffer system. A color-coded 10X NEBuffer is supplied with every restriction endonuclease, ensuring 100% activity. Over 170 restriction enzymes exhibit 100% activity in NEBuffer 4, resulting in increased efficiency, flexibility and ease of use especially when performing double digests.To help select the best conditions for HYPERLINK /nebecomm/tech_reference/restriction_enzymes/double_digests.asp double digests, this chart shows the optimal (supplied) NEBuffer and approximate activity in the four standard NEBuffers and NEBuffer EcoRI for each enzyme. If BSA is supplied with an enzyme, it is included in all NEBuffer activity reactions. In addition, the table shows recommended reaction temperature, HYPERLINK /nebecomm/tech_reference/restriction_enzymes/heat_inactivation.asp heat inactivation temperature, recommended HYPERLINK /nebecomm/tech_reference/restriction_enzymes/diluent_buffer_compatibility.asp diluent buffer and whether the enzyme is HYPERLINK /nebecomm/tech_reference/restriction_enzymes/timesaver_enzymes.asp Time-Saver qualified (i.e., cleaves substrate in 5 minutes under recommended conditions). Note: The values listed in this table are approximate. They were obtained using each enzymes specific unit assay substrate DNA. Please check out other technical reference information related to restriction enzymes:  HYPERLINK /nebecomm/tech_reference/restriction_enzymes/double_digests.asp Double Digestion | HYPERLINK /nebecomm/tech_reference/restriction_enzymes/heat_inactivation.asp Heat Inactivation | HYPERLINK /nebecomm/tech_reference/restriction_enzymes/activity_of_thermophiles_37c.asp Activity at 37°C | HYPERLINK /nebec