biochemicaltests-medicaluniversityofsouthcarolina-.pptVIP

BIOCHEMICAL TESTS PART ONE STARCH TEST Differentiates bacteria based on their ability to hydrolyze starch with the extra cellular enzyme amylase Starch a polysaccharide is to large to pass through the bacterial cell wall; to be of metabolic value to the bacteria, starch must be split into smaller fragments or individual glucose molecules Procedure: Divide one starch plate into thirds and inoculate with E.coli, Bacillus cereus, and P.aeruginosa Results: Starch and its sugar subunits are clear in the medium. Iodine is used to detect the presence or absence of starch in the vicinity around t