NucleicAcidPurificationItsPrincipleandMiniaturiz.pptVIP

Nucleic Acid Purification : Its Principle and Miniaturization MEC seminar 2004. Apr. 13 Ji Youn Lee Topics Brief introduction of nucleic acid purification principle Summary of papers about nucleic acid purification on chip Purification from What? Proteins Enzymes, DNA- or RNA-binding proteins, transcription factors… etc. Salts Buffer change Other contaminants Short DNA fragments, dNTPs… etc. Commercially available products PCR cleanup kit, gel elution kit, plasmid, genomic DNA, total RNA, mRNA purification kits Principle General Procedure How Miniaturize? Material packing (silica beads) Inc