The identification of Lea in soybean and the analysis of GmPM30 expression.docVIP

The identification of Lea in soybean and the analysis of GmPM30 expression 　　The Key Laboratory of Plant Cell Engineering and Germplasm Innovation， Ministry of Education， School of Life Sciences， Shandong University， Jinan 250100， People’s Republic of China 　　Abstract 　　LEA protein， late-embryogenesis-abundant protein， is important in response to the salt and drought stresses in plants. Here， we identified a cDNA full length of LEA from soybean （Glycine max. L） and found that LEA enhance the ability of anti-salinity in transgenic Arabidopsis thaliana. The expression of GmPM30 increases highly under salinity， cold or ABA treatment， and enhances by certain degree under drought stress. The germination rates， primary root lengths and survival rate of GmPM30 over-expression lines are obviously higher than that of the wild-type after suffering the salinity stress. Our studies displays that GmPM30-ox apparently enhances the tolerance to salinity in Arabidopsis thaliana. 　　Key words： LEA； GmPM30； salinity resistance； soybean； Arabidopsis thaliana. 　　Introduction 　　The salt stress has become the major problem in agriculture globally in recent years. The salty earth imposes restrictions on the growth and development of crop and plants， limiting the quantity of production and confining the popularization of new excellent species. Soybean （Glycine max） is the principal crop nowadays， playing an indispensable role in food supplement and energy industry. Therefore， the policies of gene engineering of constructing the new anti-salt soybean species present critical importance， which assist to reclaim the salty fields to enhance the crop production. 　　It is well documented that many genes may facilitate the salt resistance function of the plants（Sanan-Mishra et al.，2005；Tuteja et al.，2010）. In rice， OsVTE1， which encodes a tocopherol cyclase ortholog， was induced significantly by abiotic stresses such as high salt， H2O2， drought， cold and by the plant hormones ABA and sal