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Supplementary Materials and Methods Retroviral Vectors The 2C T cell clone, from which the 2C TCR was isolated, was derived from a BALB.B C.B10-H2b/LilMcd mouse immunized with the DBA/2 H-2d mastocytoma line, P815 and restimulated with BALB/c splenocytes in vitro 1. The nanomolar-affinity TCR m33 was generated from the wild-type TCR 2C by yeast display 2. Both TCR ?-P2A-? and CD8 ?-P2A-? expression cassettes 3 were optimized for expression in Mus musculus at Geneart GmbH Regensburg, Germany . The optimized TCR sequences also contained point mutations for an additional disulfide bond in t