zebrafishresearchandadultdbaclinic-diamondblackfan.org.uk.pptVIP

Hb – novel, challenging FACs – there is a phenotpye – worth exploring * * In order to understand how L-leucine increase translation in DBA we are using a novel technique called ribosome footprinting, also known as FP. It involves deep sequencing of ribosome protected mRNA fragments of about 28 nucleotides in length protected from nuclease digestion. This involves halting the ribosomes whilst they are translating and then performing a nuclease treatment to remove the overhanging mRNA leaving the protected mRNA fragments called footprints. These footprints are then recovered and converted into