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尊敬的《中国微生态学杂志》编辑：“金黄色葡萄球菌的耐药性分析及基因分型研究”一文是由鲍宏达等撰写的论著。该文通过实验分析了上海某大型医院的院内分离菌株的耐药谱和基因型，并研究了菌株的亲缘关系，对防治强致病多耐药型金黄色葡萄球菌在该院的流行提出了有针对性的意见和建议。敬请各位编辑给予指正，谢谢！文章第一作者：鲍宏达，男，1987年生，研究方向：分子流行病学通讯作者：刘畅，Email: tiantianlc@sjtu.edu.cn 我们保证该文章从未在任何公开杂志发表，也绝对没有一稿多投。金黄色葡萄球菌的耐药性分析及基因分型研究 鲍宏达 徐白莹 刘畅 韩立中 倪语星 郭晓奎 （上海交通大学医学院 上海 200025） [摘要] 目的 通过分析上海地区院内分离金黄色葡萄球菌的药敏谱型及对耐甲氧西林的金黄色葡萄球菌（MRSA）进行基因谱型的研究，了解金黄色葡萄球菌的院内流行状况。方法 对临床分离出的43株金黄色葡萄球菌进行药敏试验和SCCmec基因盒的多重PCR检测，并将结果整合后用MEGA3.1软件分析其进化相关关系。结果 药敏结果显示43株金葡菌对青霉素和甲氧西林的耐药率最高。甲氧西林的耐药率达到62.8％。MecA阳性菌株SCCmec的分型显示均为II型或III型，且所占比例相近，未见I型和IV型。进化树分析发现了在同一医院中亲缘关系相近的菌株，为院内感染流行株。结论 MecA基因介导的MRSA在分离菌株中所占比例高，存在院内感染爆发性流行。 [关键词] 耐甲氧西林的金黄色葡萄球菌、基因谱型、多重PCR、耐药性、院内感染 Typing and phylogeny analysis of methcillin-resistant Staphylococcus aureus in nosocomial infections. Hong-da BAO，Bai-ying XU，Chang Liu，Li-zhong Han, Yu-xing Ni, Xiao-kui Guo (Shanghai Jiao Tong University School of Medicine, Shanghai, 200025 China) [Abstract] Objective To determine the antimicrobial susceptibility and molecular types among clinical isolates of Methcillin-resistant Staphylococcus aureus from different departments in Shanghai Ruijin hospital, and evaluate the prevalence of Staphylococcus aureus in nosocomial infections (NI). Methods Antimicrobial susceptibility of forty-three strains isolated from patients were examined by disc diffusion method; methicillin-resistant strains with mecA gene were typed by multiplex PCR method. MEGA3.1 software was applied to analyze the homology of the strains according to the susceptibility tests and the gene typing methods. Results The rates of the resistance to penicillin and methcillin is the highest, especially the resistant methcillin is up to 62.8%. SCCmec typing method gave the result that only type II and type III strains were discovered, none of the type I and type IV were found. Relative analysis showed phylogeny homologous strains exist. Conclusion Prevalence of certain types of mecA positive MRSA strains leading to epidemic outbrea