罗汉果祛痰作用谱效关系初探-广西植物.docVIP

罗汉果祛痰作用谱效关系研究 王勤1，肖喜泉1 ，董威1，4，唐慧勤1，卢凤来3，李耀华1，李典鹏3，王剑1，林吴1、2* （1.广西中医药大学，南宁530001；2.上海全顾医疗科技有限公司，上海200120；3．中国科学院广西植物研究所，广西 桂林541006； 4．嘉兴市第一医院药剂科，浙江 嘉兴 314000） 摘要：为了研究罗汉果祛痰作用与活性成分的相关性，该研究首先测定了19批罗汉果提取物高效液相（HPLC）指纹图谱和小鼠气管酚红排泌量，找出了指纹图谱共有峰，然后用灰色关联度法确定各共有峰对祛痰效果贡献度，利用偏最小二乘法（PLS）得出了各共有峰的正负相关及贡献度。结果表明，共有峰有14个，其中关联度0.8以上的有12个峰（正相关峰为3、11、12、13号峰，负相关峰为1、2、4-10、14号峰）；11、12号峰分别为氧化罗汉果苷Ⅴ和罗汉果苷Ⅴ。因此罗汉果祛痰作用不是单一成分起作用，而是多种成分的综合作用结果；其中氧化罗汉果苷Ⅴ和罗汉果苷Ⅴ有祛痰作用，贡献率较大。 关键词：罗汉果，祛痰作用，谱效关系 DOI：10.11931/guihaia.gxzw201603010 Spectrum-effect relationship on expectorat effect of Siraitia Grosvenorii. Wang Qin1, Xiao Xi-quan1, Dong Wei1、4, Tang Hui-qin1, Lu Feng-lai3, Li Yao-hua1, Li Dian-peng3, Wang Jian1, Lin Wu1、2 * (1.Guangxi University of Chinese Medicine, Nanning 530000, 2. Shanghai Comprehensive Healthcare Technology Co.,Ltd. Shanghai 200120，3.Guangxi Institute of Botany, Chinese Academy of Science, Guilin 541006, China, 4. Department of Pharmacy, The first Hospital of Jiaxing, Jiaxing 314000 ) Abstract：nvestigate the relationship between expectoration efficacyand active components of Siraitia grosvenorii, the phenol red secretion of trachea in mice and the HPLC fingerprint of siraitia grosvenorii extract were determined. Grey relational analysis was applied to confirm the contribution degree of each common peak from HPLC fingerprints for expectoration efficacy, while partial least-squares regression (PLS) was utilized to confirm either positive or negative relationship, and identify the contribution degree of Siraitia grosvenorii extract. The results indicated that 14 common peaks were choosed in HPLC fingerprints, including 12 peaks with relation rate higher than 0.8 (NO.3, 11, 12, 13 peaks were positive correlation peaks, and NO.1, 2, 4-10, 14 peaks were negative correlation peaks), NO.11 and 12 peaks were Mogroside V oxide and MogrosideV respectively. So Expectoration efficacy of Siraitia grosvenorii is contributed by a combined action of multi-components rather than one component. Mogrosi