Th17应答型哮喘模型构建 .docVIP

构建以Th17应答为主致气道中性粒细胞增高的小鼠哮喘模型 赵燕1，冉雪梅1，黄毅2，程晓明2，王长征2，王导新1 (重庆医科大学第二附属医院呼吸内科1 ， 重庆，400010；第三军医大学新桥医院全军内科研究所2， 重庆，400037) [通信作者]王导新1，电话E-mail:wangdaoxin1@163.com [摘要]目的：建立一种TH17应答增强致气道炎症以中性粒细胞浸润为主的哮喘动物模型。方法：腹腔与气道双重致敏构建新哮喘模型。末次激发24小时后行肺功能测定，评估气道高反应性（AHR）。BALF分类计数计算气道炎症细胞比例，肺组织切片HE染色观察病理改变。Q-PCR检测肺组织TH1、TH2和 TH17细胞偏振情况。结果：腹腔与气道双重致敏可以诱发更剧烈的AHR。BALF分类计数显示嗜酸性粒细胞和中性粒细胞比例分别为16.09±4.42%和28.63±8.89%。病检示有明显的哮喘病理改变。Q-PCR结果示腹腔致敏肺内T细胞主要向TH2方向偏振，腹腔与气道双重致敏以向TH17方向偏振为主。结论：成功构建肺组织内以TH17应答占优势、气道周围炎症以中性粒细胞浸润为主的哮喘动物模型。 关键词：哮喘模型；TH17应答；气道炎症；中性粒细胞 Establishment of TH17-mediated mouse allergic asthma model with increased neutriophils around airways ZHAO Yan1,RAN Xue-mei1,HUANG Yi1, CHENG Xiao-ming2, WANG Chang-zheng2,WANG Dao-xin1 1The Seconed Affiliated Hospital of Chongqing University of Medical Sciences, Chongqing 400010;2.Xinqiao Hospital, Third Military Medical University, Chongqing 400037 Corresponding author: Wang Dao-xin1,Tel E-mail:wangdaoxin1@163.com Abstract: Objective to establishment a new TH17-mediated mouse allergic asthma model with increased neutrophils around airways. Method Sensitization through the peritoneum and airway to establish the new model .AHR tested 24 hours after the last challenge . Analysised the phenotypes of inflammation cells in the BALF. Observed the pathology changes of lung tissues. Q-PCR expressing the polarizing levels of the TH1、TH2 and TH17 cells.eutrophilic（±8.89%） major subtype of BALF inflammatory cells , then was the eosinophilic（±4.42%）Q-PCR indicated na?ve T cells main main polarizing to TH17 cells. Conclusions Successfully established TH17 cells main mediated asthma mouse model as nutrophils were major inflammatory cells around airways. Key words: asthma model; TH17-meidated response; airway inflammation; neutrophil 支气管哮喘是一种由多种细胞和细胞组分参与的气道炎症性疾病。Th1/Th2应答反应的失衡在哮喘的发病中起了很重要的作用，目前临床上用于治疗哮喘的药物也以针对Th2强应答为主。Th17是一类新型T辅助细胞，表现通过分泌IL-17诱导中性粒细胞应答。施宇衡【1】等发现支气管哮喘患者有外周血Thl7细胞及IL_17水平升高，中重度患者升高水平显著。随着对哮喘人群Thl7细胞及IL_17水平认识