坦克舱室有害气体对驾乘人员血清脂质过氧化物和抗氧化酶活性的影响.docVIP

坦克舱室有害因素对乘员血清脂质过氧化物和抗氧化酶活性的影响 孙 磊1,李代波1,王 涛2,赵晓龙1,刘晋祎1,韩 飞1,杨 桓1,刘文斌1,陶成玉3,王洪飞4,崔志鸿1,曹 佳1 (400038 重庆,第三军医大学：军事预防医学院毒理学研究所1，西南医院急救部2；233010 蚌埠,解放军装甲兵学院(P ＜0.01，P ＜0.05)，MPO活性显著高于对照组(P ＜0.01)。与同年龄段的对照组人员相比，21～25岁年龄段组MDA含量及MPO活性显著高于对照组同年龄段组(P ＜0.05，P ＜0.01)，SOD、GPx活性显著低于对照组(P ＜0.05)。与同军龄段的对照组人员相比，观察组0～2年军龄段组SOD活性显著低于对照组同军龄段组(P ＜0.05)，MPO活性显著高于对照组同年龄段组(P ＜0.01)；观察组3～5年军龄段组MDA含量和MPO活性显著高于对照组同年龄段组(P ＜0.01)。与对照组相比，不同坦克作业岗位中炮长血清MPO活性显著升高(P ＜0.01)，驾驶员血清SOD及GPx活性显著降低(P ＜0.05, P ＜0.01)，MPO活性显著升高(P ＜0.01)。结论 长期暴露于舱室有害因素的坦克乘员受到的氧化损伤加重，同时机体的抗氧化能力降低；不同年龄段、军龄及舱室岗位的乘员员中，分别以21～25岁、3～5年军龄段及坦克驾驶员受到的氧化损伤最重。 [关键词] 坦克舱室;超氧化物歧化酶;谷胱甘肽过氧化物酶;过氧化氢酶;髓过氧化物酶;丙二醛 [中图法分类号] [文献标志码]A Effects of harmful factors on lipid peroxidation and activities of antioxidative enzymes in tank occupational population Sun Lei1, Li Daibo1, Wang Tao2, Liu Jinyi1, Han Fei1, Yang Huan1, Liu Wenbin1, Tao Chengyu3, Wang Hongfei3,Cui Zhihong1, Cao Jia1 (1 Institute of Toxicology, College of Military Preventive Medicine, 2 Department of Emergency, Southwest Hospital, Third Military Medical University, Chongqing,400038; 3the Medical Team of Coaching Regiment, 4the Out-patient Department, College of Armored forces, Bengbu, Anhui Province 233010) [Abstract] Objective To explore the effects of harmful factors in tank cabins on lipid peroxidation and anti-oxidative enzymes activities of tank crews. Methods 169 soldiers occupied in tanks as the observation group and 169 soldiers without any toxicant contacted as control group in the same forces were selected in the study. The concent of malondialdehyde (MDA) and activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), myeloperoxidase (MPO) in soldiers’ serum were measured with colorimetric method. Results The activities of SOD and GPx in observation group are significantly lower than that in control group (P ＜0.01, P ＜0.05). MPO activity is significantly higher than that in control group (P ＜0.01). Compared with the co