当归多糖对辐射损伤小鼠骨髓基质细胞的作用 .doc

当归多糖对辐射损伤小鼠骨髓基质细胞的影响 关雪晶，吴 宏，何晓莉，姜 蓉 （400016重庆, 重庆医科大学干细胞与组织工程研究室，组织胚胎学教研室）[摘要] 目的当归多糖（Angelica polysaccharides ）对辐射损伤小鼠骨髓基质细胞（BMSC）的。方法 BALB/c小鼠随机分为组：正常组，照射后分别给予NS）、2 mg/kg APS和8 mg/kg APS，给药至第714、21 d计数小鼠外周血WBC）、RBC）、PLT）及BMNC）；观察BMSC生长达80%所需时间及形成的CFU-F）数量；流式细胞术检测BMSC细胞周期、凋亡率及表面黏附分子CD54、CD106的表达水平。结果 与正常组比较，NS组外周血WBC、RBC、PLT及BMNC计数明显减少；BMSC达80%所需时间明显延长，CFU-F数明显减少；G0/G1期细胞比例显著增加，S期细胞比例显著降低；BMSC细胞凋亡率明显增高；CD54、CD106表达明显降低。2 mg/kg APS和8 mg/kg APS组与NS组相比，外周血各指标BMNC数及CFU-F数明显增加；BMSC达80%融合时间G0/G1期比例降低，S期比例显著增加；凋亡率显著降低；CD54、CD106表达明显增高。第21d 8 mg/kg APS组各指标均恢复正常。结论 APS可能是通过提高BMSC贴壁及增殖能力，加速BMSC由G0/G1期向S期转换，降低BMSC凋亡率，上调CD54、CD106表达来促进辐射损伤后小鼠造血功能的恢复。 [关键词] 当归多糖辐射损伤骨髓基质细胞凋亡率[中分类号][文献标识码] AEffects of Angelica Polysaccharides on Bone Marrow Stromal Cell in Radiation Injured Mice Guan Xuejing, Wu Hong, He Xiaoli, Jiang Rong (Laboratory of Stem Cell and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China) [Abstract] Objective To investigate the radioprotective effect of Angelica polysaccharides (APS) on bone marrow stromal cell in radiation injured mice. Methods BALB/c mice were divided into ten groups at random，normal group was not radiated，the other nine groups were radiated by X-ray, then were injected with NS，2 mg/kg APS and 8 mg/kg APS. While giving different doses of APS for 6,13 ,21 days continuously to radiation injured mice, techniques of peripheral blood cells and BMNC count were made, time of 80% adherent BMSC were recorded, number of CFU-F were counted in each group, cell cycles, apoptosis ratio and the expression of CD54, CD106 on BMSC were detected by flow cytometry. Results To compare with those in normal group, the numbers of the peripheral leukocyte, erythrocyte, platelet and BMNC in NS group decreased significantly, time of 80% adherent BMSC prolonged significantly, the number of CFU-F largely decreased, the percentage of G0/G1 phases increased, whereas declined obviously in S phases, the apopto