玉米须荷叶和蒲公英萃取物护肝功能之研究.doc

番石榴葉萃取物對之 Effect of guava leaf extracts on alcohol-injured rat liver cells 研究生： Yun-Chieh Pan 指導教授：沈賜川、 Szu-Chuan Shen, Ming-Chang Wu 【摘要番石榴 Guava, Psidium guajava L. 為熱帶水果的一種，屬於桃金孃科，通常以新鮮的方式食用，也會製成加工食品，例如果汁、糖漿、果醬等。也是台灣重要的經濟作物之一。文獻指出其果實以及葉子具有止瀉，抗菌，抗氧化等功效。本研究以番石榴為實驗樣品，探討其不同溶劑之萃取方式對於大鼠正常肝細胞 clone 9 之毒性及其對酒精誘導肝損傷後的保護效果。結果顯示以番石榴萃取物處理 clone 9 細胞24小時，由細胞發現具有劑量依賴性。根據結果顯示，在細胞存活率試驗上發現水萃取物00 μg/mL以下之濃度對肝細胞並無毒性以及60℃熱水萃取物00 μg/mL以及50 μg/mL對clone 9肝細胞不具有細胞毒性。ALT之活性變化則為丙酮萃取物在200 μg/mL以下乙醇萃取物在500 μg/mL以下其ALT正常值內，番石榴葉水萃取物以及熱水萃取物組別中對細胞之。根據結果顯示應用在clone 9肝細胞之酒精損傷濃度以5％誘導30分鐘做為酒精傷肝模式，丙酮萃取物以及乙醇萃取物對以5％酒精誘導30分鐘之保護效果在100 μg/mL及50 μg/mL下較為顯著，而水萃取物在200 μg/mL其預防效果較為顯著；熱水萃取物在高濃度下即對細胞有預先保護效果。在ALT（alanine aminotransferase）活性中丙酮萃取物以及乙醇萃取物之預防效果分別在100 μg/mL及50 μg/mL以下之濃度較為顯著。而水萃取物與熱水萃取物分別在50 μg/mL以及200 μg/mL其預防效果較為顯著對於以酒精誘導之肝細胞其保護能力較佳。番石榴、、肝臟保護效果Abstract】 Guava Psidium guajava L. is a tropical fruit, which belongs to the family Myrtaceae, widely consumed fresh and also processed beverages, syrup, jams, and ice cream , it is one of the most important economic fruit in Taiwan. Leaves and fruits of guava have been reported to have anti-diarrheal, hypoglycemic, lipid lowering, antibacterial, antioxidant activities. The sample of this study was guava leaf used different solvents extraction, the cytotoxicity of clone 9 liver cells and the hepatoprotective properties of liver injuries induced by alcohol was investigated. When the clone 9 liver cells were treated with guava leaf extracts for 24 hours, the dose-dependent was found. The aqueous extracts was without cytotoxicity in 400 μg/mL, 200 μg/mL, 100 μg/mL and 50 μg/mL, the 60℃ aqueous extracts was without cytotoxicity in 100 μg/mL and 50 μg/mL. According to ALT alanine aminotransferase activities, the acetone extracts in 200 μg/mL, ethanol extracts in 500 μg/mL, 400 μg/mL, 200 μg/mL, 100 μg/mL and 50 μg/mL were normal, the aqueous extracts and 60℃ aqueous extracts were without influence. The clone 9 liver cells cultivated with 5％ alcohol for 30