白虎汤在脂肪细胞加强胰岛素促进葡萄糖吸收是经由PPARy 的作用.docVIP

PPAR-GAMMA TRANSACTIVATION-MEDIATED POTENTIATION OF GLUCOSE UPTAKE BY BAI-HU-TANG 1,2Ching-Chu Chen, 3Chien-Yun Hsiang, 4An-Na Chiang, 5Hsin-Yi Lo, 6Chia-Ing Li 1Division of Endocrinology and Metabolism, Department of Medicine, China Medical University Hospital. 2Department of Endocrinology and Metabolism, College of Chinese Medicine, China Medical University. 3Department of Microbiology, School of Medicine, China Medical University. Taichung, Taiwan; 4Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei, Taiwan; 5Department of Nuclear Medicine and PET Center, 6Department of Medical Research, China Medical University Hospital, Taichung, Taiwan. Background and Aim: Plant derivatives have been used to reduce hyperglycemia in folk medicine around the world for centuries. We performed a 3T3-L1 adipocyte-based glucose uptake screening assay to measure the glucose uptake effects of herbal medicines that can be potentially used to treat patients with type 2 diabetes mellitus. We found that Bai-Hu-Tang BHT , a traditional Chinese formula, composed of anemarrhena, gypsum, licorice and rice, was able to stimulate glucose uptake in 3T3-L1 adipocytes. The aims of this study were to explore the glucose uptake effect as well as its mechanism s of action of BHT. Material and Method: Differentiated 3T3-L1 adipocytes were treated with vehicle, insulin or insulin plus different concentrations of BHT. The 2-deoxy-[3H] glucose uptake assay was performed to measure the amount of glucose uptake. To explore the mechanism s of glucose uptake of BHT, we used PI3 kinase inhibitor and CAP inhibitor to test if the glucose uptake effect was mediated by the insulin signaling pathway. We then used Western blot analysis to re-confirm the result of the insulin signaling inhibition assay. Finally, reporter chimera assay of HuH-7 cells was used to measure the PPARγ activation by BHT. Results: We found that BHT potentiated insulin-stimulated glucose uptake in 3T3