粗肋草细菌性软腐病病原菌之监定及其病害防治之研究.docVIP

粗肋草細菌性軟腐病病原菌之鑑定及其病害防治之研究 Etiology and control of aglaonema bacterial soft rot 研究生：馮靖廷 Feng, Ching-Ting 指導教授：何婉清 Ho, Wang-Ching 【摘要】 2003年6月於屏東縣內埔鄉中林村金獅園藝，發現自泰國進口之”彩虹 Sithiporn aglaonema ”品種粗肋草植株發生嚴重軟腐，分離出7株’s postulates 後，經modified crystal violet pectate CVP 選擇性培養基、馬鈴薯薯片及白菜菜梗接種等步驟，進行病原細菌初步鑑定，確認所分離出之細菌皆為Pectobacterium軟腐細菌，經傳統生理生化測定並配合專一性引子對5A/5B，以聚合酶連鎖反應 PCR 進行種的鑑定，結果顯示所分離出之7株軟腐細菌皆為P. chrysanthemi。P. chrysanthemi菌株，分別以ASR1至ASR7命名，經接種至”彩虹”粗肋草，證實ASR3菌株的致病力最強，接著並以彩虹品種粗肋草切離葉接種法抗病篩選之可行性，結果顯示彩虹粗肋草切離葉於接種處會產生典型的軟腐病，並植株活體葉片接種，，說明離葉接種法可做為粗肋草葉部病害抗病篩選的替代方法。溫度可影響粗肋草細菌性軟腐病之發生，20至32℃造成細菌性軟腐病的危害，其中以2℃為最適發病溫度，當接種濃度在104 cfu/ml以上時，即能快速發病，病勢會隨著接種濃度之增加而越趨嚴重。以分離之7株軟腐細菌菌株進行其對不同收穫後農產品之致腐能力測試，菌株，結果顯示本研究室收集之16株木黴菌菌株T1、T4、T5、T6、T13、T14、T17等菌株抗生物質。再以離試防治效果，結果顯示木黴菌T1之培養過濾液，而。關鍵詞：粗肋草 Aglaonema 、細菌性軟腐病、Pectobacterium菌、木黴菌 Trichoderma 、螢光假單胞菌、防治In 2003, soft rot symptoms on Sithiporn aglaonema Aglaonema tricolor × A. nitidium leaves were found in a nursery at Neipu, Pingtung, in Taiwan. 7 isolates of bacteria from soft rot aglaonema tissues could cause the typical soft rot symptoms both on aglaonema plants and the detached aglaonema leaves by inoculating the bacterial suspensions of those 7 isolates with a concentration of 108 cfu/ml on the carborumdum wounded tissues. No symptoms on control plants and leaves were showed when they were inoculated with sterilized distilled water. Bacteria could be reisolated from the inoculated tissues that fulfilled the Koch’s postulated. All 7 isolates of the bacteria were proven belong to soft rot Pectobacterium, due to they were negative for gram stain. Could depredated the pectate on CVP medium and could cause soft rot symptoms on potato slices and Chinese cabbage petioles. According to Dickey Kelman and Goto Matsumoto, the physiological characters of these 7 isolates matched the characters of P. chrysanthemi. The primer pair 5A/5B also could further confirm that all isolates were P. chrysanthemi by polymerase chain reaction PCR . All isolates were, the