水稻类病斑突变体lmm4的鉴定及其基因定位教程范本.doc

水稻类病斑突变体lmm４的鉴定及其基因定位 邱结华１　马宁１,２　蒋汉伟１,２　圣忠华１　邵高能１　唐绍清１　魏祥进１,　胡培松１, (１中国水稻研究所/农业部水稻生物学与遗传育种重点实验室/水稻生物学国家重点实验室,杭州３１０００６;２杭州师范大学生命与环 境科学学院,杭州３１００３６;通讯联系人,EＧmail:weixiangjin＠caas．cn;hupeisong＠caas．cn) IdentificationandGeneMappingofaLesionMimicMutantlmm４inRice QIUJieＧhua１,MANing１,２,JIANGHanＧwei１,２,SHENGZhongＧhua１,SHAOGaoＧneng１,TANGShaoＧqing１, WEIXiangＧjin１,,HUPeiＧsong１, (１StateKeyLaboratoryofRiceBiology,KeyLaboratoryofRiceBiologyandBreedingofMinistryofAgriculture,ChinaNational RiceResearchInstitute,Hangzhou３１０００６,China;２CollegeofLifeandEnvironmentalSciences,HangzhouNormalUniversity, Hangzhou３１００３６,China;Correspondingauthor,EＧmail:weixiangjin＠caas．cn;hupeisong＠caas．cn) QIUJiehua,MANing,JIANGHanwei,etal．Identificationandgenemappingofalesionmimicmutantlmm４inrice． ChinJRiceSci,２０１４,２８(４):３６７Ｇ３７６． Abstract:Thelesionmimicmutantlmm４wasidentifiedfromanEMSＧinducedNipponbaremutantlibrary．Brown lesionswerefirstobservedonthetipoflmm４leavesattheinitialtilleringstage,andspreadedgraduallydownwardto thewholeleafbladeandeventheleafsheath．Comparedwiththewildtype,thechlorophyllandthenetphotosynthetic rateoflmm４weresignificantlyreduced．Theplantheight,paniclelength,seedsettingrate,grainweightwerealso decreasedsignificantly．Theshadingassayshowedthatthelesionsonlmm４leaveswereinducedbynaturallight．The histologicalstainingassayindicatedthatthelesionsonlmm４leaveswerecausedbyprogrammedcelldeathand hydrogenperoxideaccumulatedinlmm４leaves．Thelmm４displayedhigherresistancetoblastraceC７andG１when comparedwiththewildtype．Inaddition,pathogenesisＧrelatedgenePOC１,PAL,PBZ１,PR１werefoundtobeupＧ regulatedinlmm４．Geneticanalysissuggestedthatthephenotypeoflmm４wascontrolledbyasinglerecessivenuclear locus．BasedamapＧbasedcloningstrategy,thelmm４wasnarroweddowntoa２３５kbregionbetweenIndＧ４andIndＧ６ nearthecentromereofchromosome５． Keywords:lesionmimicmutant;blastresistance;genemapping;rice 邱结华,马宁,蒋汉伟,等．水稻类病斑突变体lmm４的鉴定及其基因定位．中国水稻科学,２０１４,２８(４):３６７Ｇ３７６． 摘　要:在EMS诱变的日本晴突变体库中,筛选到一个类病斑突变体.该突变体类病斑出现于分蘖始期,而后慢慢扩散至 叶鞘、茎秆,最后至整个植株,属于扩散型