园艺植物生物技术第四章研究报告.ppt

Basic procedures of enzymolysis method Culture methods Plate culture 平板培养 Nurse culture看护培养 Microculture微室培养 Conditioned medium culture条件培养基培养 The cells in culture exhibit the following five phases of a growth cycle i. Lag phase, where cells prepare to divide. ii. Exponential phase, where the rate of cell division is highest. iii. Linear phase, where cell division slows but the rate of cells expansion increases. iv. Deceleration phase, where the rates of cell division and elongation decreases. v. Stationary phase, where the number and size of cells remain constant. 2. Protoplast Isolatio