分子生物实验作.pptVIP

Influence of RT-qPCR primer position on EGFR interference efficacy in lung cancer cells主题-肺癌细胞中在表皮生长因子受体干扰效能上RT-qPCR引物位置的影响。 Abstract Background: Real-time quantitative RT-PCR (RT-qPCR) is a “gold” standard for measuring steady state mRNA levels in RNA interference assays. The knockdown of the epidermal growth factor receptor (EGFR) gene with eight individual EGFR small interfering RNAs (siRNAs) was estimated by RT-qPCR using three different RT-qPCR primer sets. Results: Our results indicate that accurate measurement of siRNA efficacy by RT-qPCR requires careful attention for the selection o