NCBI-3.ppt

NCBI介绍 同济医学院 公共卫生学院 王友洁 wangyoujie@mails.tjmu.edu.cn Genbank的历史 最早建立于1979年，由Los Alamos National Laboratory的研究团队建立和维护 在1983年，有2000多条序列记录存贮在Genbank 1989-1992年Genbank 由刚建立的NCBI维护 2014年2月15日，有171,123,749条序列记录 GenBank GenBank? is a comprehensive database that contains publicly available nucleotide sequences for over 280 000 formally described species. These sequences are obtained primarily through submissions from individual laboratories and batch submissions from large-scale sequencing projects, including whole-genome shotgun and environmental sampling projects. (Nucleic Acids Res.?2014 Jan;42(Database?issue):D32-7.? ) Growth of Genbank Sequence identifiers and accession numbers Each GenBank record, consisting of both a sequence and its annotations, is assigned a unique identifier called an accession number that is shared across the three collaborating databases (GenBank, DDBJ, EMBL). The accession number appears on the ACCESSION line of a GenBank record and remains constant over the lifetime of the record, even when there is a change to the sequence or annotation. Changes to the sequence data itself are tracked by an integer extension of the accession number, and this?Accession.version?identifier appears on the VERSION line of the GenBank flat file. The initial version of a sequence has the extension ‘.1’. In addition, each version of the DNA sequence is also assigned a unique NCBI identifier called a?GI?number that also appears on the VERSION line following the Accession.version: ACCESSION?AF000001 VERSION?AF000001.1?GI : 987654321 Direct electronic submission Submission using BankIt convenient and quick submission of sequence data Submission using Sequin the output files for direct submission should be sent to GenBank by e-mail. Submission using Sequin and tbl2asn It is used primarily for submission of complete genomes and large batches of sequences. GenBank数据库检索 核苷酸序列数据库分为三个子数据库： EST :表达序列标记数据库 GSS :基因组测序序列数据库 Nucleotide :包含所有未被以上两个子数据库收