mycobacterium.pptVIP

Mycobactrium Tuberculosis(结核分枝杆菌) 1.?morphology staining *thin, straight or slightly curved rods *thick, lipid-rich cell wall *non-motile, non-spore, non-capsule 2.?culture *obligate aerobe *special nutrient requirement *slow growth *colony -- “cauliflower” *pellicle on surface of liquid media 3.resistance *resistant to dry (in dried sputum, 6-8 months) acid -- 3% HCl, 6% H2SO4 alkali -- 4% NaOH dyes *sensitive to moist heat disinfectant -- alcohol drug -- rifampin, isoniazid, etc UV 4.Variation II Pathogenicity Immunity cellular mediated immunity (CMI) and delayed type hypersensitivity (DTH) of host greatly influence the development of the disease. CMI： CD4 positive T cells and activated macrophages. DTH: Tuberculin Skin Test PPD injection(PPDC, BCGPPD 5u) 48-72h induration, erthyema “-” --- ? 5mm: # no TB infection; # early stage of infection; # severe patient suffered with TB; # the level of cellular immunity is low: tumor, AIDS, use immunosuppressive agent “+” --- 5mm ? ? 15mm: hypersensitive to M. tuberculosis: BCG Previously Infected “++”--- ? ? 15mm: active TB 3. application * detect immunity effect of BCG inoculation * epidemiological investigation of TB infection * help to diagnosis TB 1. specimen: sputum, urine etc. * direct smear ---- acid-fast stain * treatment with NaOH and concentration by centrifugation---- acid-fast stain 1.Pathogenic factor (1)?Lipid (closely related to virulence) cord factor phosphatide wax D sulfatides cord factor 1). binds to mitochondrial membrane, cause f