Supplementary Information Letter to the Editor.docVIP

Supplementary Information Letter to the Editor Levels of glucocorticoid receptor and its ligand determine sensitivity and kinetics of glucocorticoid-induced leukemia apoptosis Georg Gruber1,2, Michela Carlet1,2, Elisabeth Türtscher2, Bernhard Meister2,3, Julie A. E. Irving4, Christian Ploner1, and Reinhard Kofler1,2 1Division Molecular Pathophysiology, Biocenter, Medical University of Innsbruck, 6020-Austria 2Tyrolean Cancer Research Institute, Innsbruck, 6020-Austria 3Department of Pediatrics, Medical University of Innsbruck, 6020-Austria 4Northern Institute for Cancer Research, Newcastle upon Tyne, UK Correspondence: Prof. Reinhard Kofler, M.D. Division of Molecular Pathophysiology, Biocenter, Medical University of Innsbruck Fritz-Pregl-Stra?e 3, A-6020 Innsbruck, AUSTRIA Tel: 0043-512-9003-70360, Fax: 0043-512-9003-73960; e-mail: Reinhard.Kofler@i-med.ac.at Table of Contents: Supplemental Figure S1 to S3…………………………………………………………….. 2 Materials and Methods ……………………………………………………………………. 5 References ………………………………………………………………………………… 7 Materials and Methods Patients, cell lines and tissue culture The characteristics of 2 children with T-cell ALL (T-ALL-20, T-ALL-25) and 1 with precursor B-cell ALL (B-ALL-40) have been detailed previously2. B-ALL-19 and B-ALL-44 derive from the same cohort of ALL children (Medical University of Innsbruck Ethics Committee # EK1-1193-172/35) but have not been published previously. B-ALL-19 was a 14-year old boy, B-ALL-44 a 6.5-year old girl, both with CD10-positive precursor B-ALL. In all 5 instances, 80% pure peripheral blood lymphoblasts were used. T-ALL-20 cells were 90% pure and hence were not further enriched. T-ALL-25 and B-ALL-19 cells were enriched by negative and B-ALL-40 and -44 by positive selection using anti-CD10 antibodies as described2. All 5 patients showed a decrease in peripheral blasts within the first 24h and patients responded well to the initial systemic GC monotherapy as defined by the BFM protocol, i.e., t