系统生物学软件-3.pptVIP

* * * * Define a threshold, Δ. Find the smallest positive d(i) such that call it t1. In a similar manner, find the largest negative d(i). Call it t2. For each gene i, if, call it potentially significant. Identifying Significant Genes * * SAM Output List of significantly changing genes Fold changes may be asymmetric Estimated false positive rate for the list * Where are these genes? * SAM- procedure overview Sample genes expression scale Define and calculate a statistic, d(i) Generate permutated samples Estimate attributes of d(i)’s distribution Identify potentially Significant genes E