青花菜及近缘种属种质资源抗根肿病鉴定.docVIP

青花菜及近缘种属种质资源抗根肿病鉴定 张小丽 刘玉梅＊ 方智远 杨丽梅 庄 木 张扬勇 李占省 吕红豪 （中国农业科学院蔬菜花卉研究所，北京100081） 摘要:根肿病是十字花科蔬菜作物的主要病害之一，造成蔬菜产业巨大经济损失。青花菜是一种重要的十字花科蔬菜，具有良好的防癌保健功效。近年来青花菜根肿病在我国浙江、云南等青花菜主产区发生日益严重，鉴定和筛选青花菜抗根肿病抗源，从而培育抗病品种是防治该病最经济有效的方法。为挖掘和丰富可利用的根肿病抗源，本试验针对我国优势根肿菌小种—4号小种，利用苗期人工接种鉴定方法—伤根灌菌法对531份青花菜及其近缘种属材料进行了抗根肿病鉴定。结果显示，446份青花菜材料（其中高代自交系393份，杂交种53份）中缺乏高抗(HR)和抗病(R)材料；中抗（MR）材料5份，均为自交系，占供试种质的1.12%；感病（S）材料189份，占供试种质的42.38%；高感（HS）材料252份，占供试种质的56.50%；85份近缘种属材料（其中甘蓝9份，花椰菜32份，大白菜7份，芜菁4份，芥蓝12份，苤蓝8份，菜心8份，油菜2份，野生种2份，欧洲山芥1份）中，免疫（I）材料1份，高抗（HR）材料 1份，抗病（R）材料 5份，中抗（MR）材料2 份，感病（S）材料39份，高感（HS）材料37份。总体上高抗病青花菜材料缺乏，近缘种属材料中抗源材料比例略高，这些材料为青花菜及甘蓝类蔬菜抗根肿病病育种提供了抗性资源。 Identification of Germplasm Resistant to Clubroot in Broccoli and Its Relatives Zhang Xiaoli Liu Yumei* Fang Zhiyuan Yang Limei Zhuang Mu　Zhang Yangyong Li Zhansheng Lv Honghao (Institute of Vegetables and Flowers of Chinese Academy of Agricultural Sciences, Beijing 100081, China ) Abstract: Clubroot is a destructive disease??of?cruciferae?vegetable?crops,?which?causes?great?economic?loss.? Broccoli?is an important?cruciferae?vegetable,?which?has? good anti-cancer effects. In recent years, the incidence of clubroot is increasing in the main producing areas of broccoli, such as Zhejiang, Yunnan province. Cultivating resistant varieties based on identifying and screening clubroot-resistance accessions is the most cost-effective method of preventing the disease. To?explore?available?resistant germplasm aganinst NO.4 race, the mainstream Plasmodiophora brassicaerace race in china, 531?broccoli?accessions and their relatives were artificial?inoculated using irrigating injured root method. The?results?suggested?that?5?accessions?(inbred?lines) were middle resistant (MR),?accounting?for?1.12%?; 189 accessions?were susceptible??(S), accounting?for?42.38%; 252?accessions?were?highly susceptible (HS), ?accounting?for?56.50%; no accessions were highly?resistant?(HR)?or?resistant?(R)?among 446 broccoli accessions (including 393 inbred?lines,?53?hybrids). Amo