广西火桐基因组DNA提取方法的研究.docVIP

广西火桐基因组DNA提取方法的研究 　　摘要：以广西火桐[Erythropsis kwangsiensis （Hsue） Hsue]叶片为试料，比较了改良CTAB法、试剂盒法和改良SDS法提取基因组DNA的提取效果及对干燥叶和鲜叶提取的基因组DNA进行了比较研究。结果表明，改良CTAB 法和试剂盒法提取的基因组DNA质量较好、杂质少，适合广西火桐基因组DNA的提取。改良SDS法提取的基因组DNA无完整条带，质量较差，不适合广西火桐基因组DNA提取。硅胶干燥90 d的叶样和新鲜叶样所得的基因组DNA纯度相当，产率前者多于后者。 　　关键词：广西火桐[Erythropsis kwangsiensis （Hsue） Hsue]；基因组DNA；提取 　　中图分类号：Q781 文献标识码：A 文章编号：0439-8114（2013）20-5060-03 　　Studies on Genomic DNA Extraction of Erythropsis kwangsiensis 　　LUO Wen-hua1，HUANG Shi-xun1，MA Hu-sheng1，FU Zhi-hong1，2，DAI Wen-juan1， 　　TANG Wen-xiu1，PAN Bo1，ZHAO Bo1 　　（1.Guangxi Institute of Botany， Guangxi Zhuang Autonomous Region and the Chineses Academy of Sciences， Guilin 541006，Guangxi， China； 2. College of Life Sciences， Guangxi Normal University， Guilin 541004，Guangxi， China） 　　Abstract： Using the leaves of Erythropsis kwangsiensis as material， the genomic total DNA was extracted. Three methods of genomic total DNA extraction including improved CTAB method， reagent box method and improved SDS method were compared. Results showed that the DNA extracted from improved CTAB method and reagent box method had high quality and less impurity， but quantity of the latter was less than that of the former. The reagent box method had the advantages of simple operation， short time and less harmful， but more expensive than that of the improved CTAB method. In addition， improved SDS method didn’t have the complete bands， so it was not suitable for genomic DNA extraction of Erythropsis kwangsiensis. The genomic DNA extracted from the leaves preserved in silica gel for 90 days were not significantly different from that of fresh leaves， and the yield of the former was much more than that of the latter. 　　Key words： Erythropsis kwangsiensis； genomic DNA； extraction 　　广西火桐[Erythropsis kwangsiensis （Hsue） Hsue]隶属梧桐科火桐属（Erythropsis）植物，为中国特有种，仅分布于广西壮族自治区中部至南部的石灰岩地区，对研究植物区系和植物地理及亲缘关系等均有重要的学术价值。其木材纹理直，材质柔韧，不开裂，是制作家具的上等用材；其先花后叶，花色鲜艳靓丽，花期长，具有极高的经济和观赏价值。由于人类的过度利用，广西火桐野外种群和个体数量稀少，已处于濒危状态，为国家二级重点保护植物[1]。目前