細胞传代培养与MTT法检测化学药品对体外培养细胞增殖及存活的影响.docxVIP

細胞传代培养与MTT法检测化学药品对体外培养细胞增殖及存活的影响.docx

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細胞传代培养与MTT法检测化学药品对体外培养细胞增殖及存活的影响

Cellular subculture and the effect of MTT assay of scanning of peroxide to the growth and survival of cells in vivo. Hu Boqiang1 Dong ZhiWei11 1School of Life Science, Beijing Institute of Technology, Beijing 100081, China Cellular subculture assays is the rudimental method of all cellular experiments. Via subculture, cells could survive, duplicate and preserve their own genes. In our experiment, we transferred the subculture cells to 96-well plate, treating with H2O2 in different concentrations and dying living cells (6251 cell line) with MTA. Finally, we got the effect of H2O2 in different concentrations to the surviving and growth of 6251 cells. 1. Introduction Hydrogen peroxide (H2O2) is the simplest peroxide (a compound with an oxygen-oxygen single bond). It can generate inside the cell. Furthermore, it is also a strong oxidizer (According to Wikipedia, item Hydrogen peroxide) which can fatal to the survival of Eukaryotic cells. However, thanks to Catalase (CAT), the enzyme which could reduce H2O2, cells could survival in vivo. Although CAT could reduce the side effect of H2O2, excessive concentration of H2O2 can cause serious diseases to people, especially in aged groups whose CAT activity were lower than the young ones. Paradoxically, H2O2 plays a “double-edge blade” role in the pathology of cancer. On the one hand, it can intervene cellular DNA repairment, intracellular signals transfer pathway, the adherence of the cell and promoting the expression of VEGF, which could accelerate the spread of tumor. On the other hand, however, H2O2, the indicator of Oxidative Stress, can induce to cell apoptosis and depress the duplication of cells. So doctors usually take advantage of H2O2, like Radiotherapy (using Ultraviolent to produce H2O2 and other peroxide) to try to cure cancer (YaoZhong, 2006). Peroxide can be found everywhere in daily life. For example, in hair dye, peroxide can make hair more flexible by cutting up the disulfide bonds betwe

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