舞茸中一種新型抗HSV-1蛋白的分离鉴别及其功能确定.docxVIP

声明：本文摘自Antiviral Research 75 (2007) 250-257，中文名《抗病毒研究》，是一本关于病毒性疾病预防和治疗研究的杂志，由国际抗病毒研究协会（ISAR）官方出版，主要收录与人类及动物病毒感染控制相关的研究报告，综述以及评论等。为方便广大读者阅读，我们对论文摘要进行了中文翻译，译文仅供参考，如有不足之处，敬请指正。本文摘自舞茸D-fraction官方网站【参考译文】???????????????????? Isolation, identification and function of a novel anti-HSV-1 protein??????????????????????????????????????????????????? from Grifola frondosa??????????????????????? 舞茸中一种新型抗HSV-1蛋白的分离、鉴别及其功能确定??????????????????????????????????????????????????????????????? 摘? 要??? 用40%硫酸铵沉淀法和DEAE纤维素离子交换色谱法从舞茸子实体中提取一种新型的抗病毒蛋白，并命名为GFAHP。这种蛋白质能在体外抑制1型单纯疱疹病毒（HSV-1）的复制，其50%抑制浓度（IC50）为4.1μg/ml，治疗指数29.3。在小鼠模型中，更高浓度的GFAHP（125和500μg/ml）也能显著减轻由HSV-1诱导的睑缘炎、新血管形成以及基质角膜炎。小鼠眼角膜外用GFAHP能显著减少病毒的产量（实验组为3.4log10PFU，对照组为4.19log10PFU）。我们证实，GFAHP在阻止HSV-1穿透进入肾细胞的同时能直接使HSV-1失活。凝胶电泳显示GFAHP的分子量为29.5kDa。用胰蛋白酶水解GFAHP后，从MALDI-TOF-MS测得的肽质量指纹谱（PMF）和电喷雾质谱法分析得知GFAHP的N端序列是一个含11个氨基酸的多肽，即NH2-REQDNAPCGLN-COOH,这与任何一种已知的氨基酸序列不匹配，这说明GFAHP很有可能是一种新型的抗病毒蛋白。据我们所知，这是关于舞茸的抗HSV蛋白的首个报道。关键词：舞茸；蛋白质；抗病毒活性；鉴定；HSV-1论文原文???????????????????????????????????????????Antiviral Research 75 (2007) 250–257??????????Isolation, identification and function of a novel anti-HSV-1 protein from Grifola frondosa??????????????? Chang-Qing Gu, Jun-Wen Li? , Fuhuan Chao, Min Jin, Xin-Wei Wang, Zhi-Qiang Shen??????????????????????? Institute of Environment and Health, 1 Dali Road, Tianjin 300050, PR China?????????????????????????????????????? Received 15 November 2006; accepted 22 March 2007Abstract? A novel antiviral protein was purified from an extract of Grifola frondosa fruiting bodies using a procedure that included 40% ammonium sulfate precipitation and DEAE-cellulose ion exchange chromatography, and designated GFAHP. This protein inhibited herpes simplex virus type1 (HSV-1) replication in vitro with an IC50 value of 4.1μg/ml and a therapeutic index 29.3. Higher concentrations of GFAHP (125 and 500μg/ml) also significantly reduced the severity of HSV-1 induced blepharitis, neovascularization, and stromal keratitis in a murine