黄芩中黄芩苷的交提取工艺及抑菌活性研究.docVIP

黄芩中黄芩苷的正交提取工艺及抑菌活性研究 杨 明，陶 蕾2, 4，王 文3，赵 强4，陈 虹4 (1.甘肃省畜牧兽医研究所，甘肃 平凉 744000；2.中国农业科学院 兰州畜牧与兽药研究所，甘肃 兰州 730050； 3.甘肃农业大学 草业学院，甘肃 兰州 730070；4.甘肃农业大学 动物医学院，甘肃 兰州 730070) 摘 要：采用正交提取法提取黄芩中黄芩苷，将提取物进行HPLC检测，并对两种供试菌进行体外最小抑制浓度（MIC）测定。结果表明：最佳正交提取工艺为A2BC3，即乙醇浓度70%、乙醇体积100mL、冷浸时间36h；对大肠埃希氏杆菌、金黄色葡萄球菌的MIC分别为：0.0 mg/mL和0.0 mg/mL。 关键词：黄芩苷正交提取抑菌 The Optimization of Extraction Process by Orthogonal Test and Invitro Antibacterial Activity for Baicalin Yang Ming, Tao lei , Wang Wen, Zhao Qiang, Chen Hong （1.Gansu Institute of Animal and Veterinary Sciences, Pingliang Gansu,744000；2. Lanzhou Institute of Animal and Veterinary Pharmceutical Sciences,Chinese Academay of Agricultral Sciences, Lanzhou Gansu, 730050；3 . Pratacultural College of Gansu Agricalture Univesity, Lanzhou Gansu,730070；4. College of Vetetinary Medicine at Gansu Agricalture Univesity, Lanzhou Gansu,730070） To optimize the extract conditions by orthogonal test for baicalin of Scutellaria baicalensi. the contents of baicalin were determined by HPLC and minimal inhibitory concentration(MIC) were measured by means of bacteriostasis tests in vitro on two kinds of bacteria to the baicalin.The results showed that The optimum extracting conditions were A2B2C3, that was the cold-maceration, 70% alcohol 100mL as the solvents and to extract for 36h; MIC of the baicalin against Escherichia coli and Staphylococcus aureus was 0.50 and 0.20 mg/mL respectively. Key words: baicalin; extraction process by orthogonal test; invitro antibacterial activity 黄芩为唇行科植物，味苦、性寒。具有清热燥湿，泻火解毒，止血、安胎的功效[1、6]。主治湿温、暑温胸闷呕恶、湿热痞满、泻痢、黄疸、肺热咳嗽、高温烦热、胎动不安等症。黄芩是祖国医学中常用中药，来源丰富、价廉。黄芩苷是黄芩的主要有效成份，本实验主要通过正交试验，探究出黄芩中黄芩苷提取的影响因素，优化实验设计，并将提取出的黄芩苷对供实菌进行抑菌实验，以为相关疾病的防治提供理论依据。 1 材料与方法 1.1 材料 1.1.1 药材 黄芩粉末（购买于甘肃省平凉市药材站，经自然风干，粉碎）。 1.1.2 仪器设备及药品 RE52-99?旋转蒸发器、ZDP-2120型电热培养箱、UV-9200型紫外-可见光分光光度计、电子分析天平。95%乙醇、甲醇、黄芩苷标准品、乙腈、甲酸、三重蒸馏水等，其余试剂均为国产或进口分析纯。 1.1.4 培养基及菌种 牛肉膏蛋白胨培养基，按规定方法配制，分装灭菌备用。大肠埃希氏杆菌（Escherichia coli，ATCC35216）、金黄色葡萄球菌（Staphylococcus aureus，ATCC26111）为甘