Inflammatoy mediators induced by coarse.docVIP

Inflammatory mediators induced by coarse (PM2.5
/10) and fine (PM2.5) urban air particles in RAW 264.7 cells 炎症介质诱导的粗（可入肺颗粒物／10）和细（可入肺颗粒物）在RAW 264.7细胞城市空气中的颗粒 Roberta Pozzi a, Barbara De Berardis b, Luigi Paoletti b,Cecilia Guastadisegni(罗伯塔，巴巴拉德路易吉保莱蒂berardis?B，B，塞西莉亚guastadisegni) a,a Laboratory of Environmental Hygiene, Istituto Superiore di Sanita`, Viale Regina Elena 299, 00161 Rome, Italy b Laboratory of Ultrastructure, Istituto Superiore di Sanita` , Viale Regina Elena 299, 00161 Rome, Italy Received 4 April 2002; accepted 19 August 2002 实验室环境卫生，卫生的Istituto?Superiore di?`，Regina埃琳娜大街299，罗马00161，意大利B实验室的超微结构，Istituto?Superiore di?`Viale?Regina埃琳娜卫生，299，00161罗马，意大利 收到四月4日2002；接受八月192002 Increased incidence of mortality and morbidity due to cardiopulmonary complications has been found to associate with elevated levels of urban air particles with an aerodynamic diameter /10 micron, PM10 and /2.5 micron, PM2.5. 由于心肺并发症的死亡率和发病率的增加已被发现与城市空气颗粒物水平升高与空气动力学直径小于10微米，PM10和＜／2.5微米，可入肺颗粒物。 Respirable particles reach the lower respiratory tract where they are phagocytized by alveolar macrophages. Depending on particle composition, exposed macrophages may produce inflammatory mediators. A cascade impactor sampler was used to collect size-fractionated urban air particles. Particulate matter from the city of Rome (Italy) were collected onto stainless steel plates, and recovered using alcohol. The murine monocytic/macrophagic RAW 264.7 cell line was used to compare the ability of PM2.5-/10, PM2.5 and carbon black to cause cell injury, such as arachidonic acid (AA) release,tumour necrosis factor alpha (TNFa) and interleukin (IL)-6 production. 可吸入颗粒物进入下呼吸道的肺泡巨噬细胞吞噬，他们是。根据颗粒组成，暴露的巨噬细胞产生炎症介质。级联撞击采样器采集城市空气颗粒大小分级。从罗马市大气颗粒物（意大利）收集到的不锈钢板，并恢复使用酒精。小鼠单核细胞/巨噬细胞RAW 264.7细胞系被用来比较的能力可入肺颗粒物pm2.5-/10，炭黑，导致细胞损伤，如花生四烯酸（AA）的释放，肿瘤坏死因子-α（TNF-α）和白细胞介素（IL）6产生。 All test particles have been used at the same concentrations 30 and 120 mg/ml. Treatment with PM2.5-/10 and PM2.5 induced significant AA rel